Laminin and a basement membrane extract have different effects on axonal and dendritic outgrowth from embryonic rat sympathetic neurons in vitro.

Developmental Biology
P J Lein, D Higgins

Abstract

We have characterized the effects of laminin and a basement membrane extract (BME) on the morphology of embryonic rat sympathetic neurons maintained in tissue culture in the absence of nonneuronal cells. Neurons were grown on polylysine-coated coverslips in the presence or absence of laminin or BME in serum-free medium. Axons were distinguished from dendrites using intracellular dye injections, immunocytochemistry, and [3H]uridine autoradiography. In short-term (less than or equal to 24 hr) culture, laminin had a potent neurite-promoting effect, causing increases in the number of processes, total neuritic length, and neuritic branching. In long-term (3-35 days) cultures chronically exposed to laminin, most (greater than 75%) neurons maintained supernumerary axons but failed to form dendrites. In contrast, most neurons (greater than 70%) grown in long-term culture on polylysine in the absence of laminin were unipolar, extending a single axon. BME caused sympathetic neurons to extend multiple (range, 1-15) dendrites. Morphometric measurements made after 1 month of exposure to BME indicated that the amount of dendritic growth that occurred in vitro was similar to that normally occurring during a comparable period in situ. BME did ...Continue Reading

References

Oct 1, 1979·The Journal of Comparative Neurology·G A Banker, W M Cowan
Feb 1, 1976·The Journal of Cell Biology·L Landmesser, G Pilar
Jan 28, 1986·Biochemistry·H K KleinmanG R Martin
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A S AschR L Nachman
Jan 1, 1987·Molecular Neurobiology·A D Lander
Jun 1, 1986·The Journal of Cell Biology·D J CareyC M Rafferty
Jan 1, 1986·Annals of the New York Academy of Sciences·L I BinderL I Rebhun
Sep 4, 1987·Science·R Levi-Montalcini
Jan 1, 1987·Annual Review of Cell Biology·G R Martin, R Timpl
Jan 1, 1987·Annals of the New York Academy of Sciences·H K KleinmanL Luckenbill-Edds
Jan 1, 1986·The Journal of Cell Biology·I PengM M Black
Feb 1, 1986·Developmental Biology·S L RogersS C McLoon
Jan 1, 1986·Annual Review of Neuroscience·R P BungeC F Eldridge
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L A Sternberger, N H Sternberger
Dec 1, 1983·The Journal of Cell Biology·M ManthorpeS Varon

❮ Previous
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Citations

Apr 1, 1991·Journal of Cellular Physiology·B S WeeksH K Kleinman
May 1, 1992·Journal of Cellular Physiology·J P LacourB A Gilchrest
Dec 15, 1993·Journal of Neuroscience Research·S Liang, K A Crutcher
Dec 1, 1992·Neurochemical Research·A CestelliI Di Liegro
Jun 21, 1991·Brain Research. Developmental Brain Research·P J Lein, D Higgins
Jun 1, 1996·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·P LeinD Higgins
Oct 1, 1991·Current Opinion in Cell Biology·I de Curtis
Mar 3, 1998·Progress in Neurobiology·T Cowen, I Gavazzi
Mar 1, 1997·The International Journal of Biochemistry & Cell Biology·S K Powell, H K Kleinman
Oct 2, 1998·Alcoholism, Clinical and Experimental Research·P A Clamp, T A Lindsley
Jun 22, 2011·Acta Physiologica·E Storkebaum, P Carmeliet
Jun 1, 1990·The Journal of Pharmacy and Pharmacology·T G Smart
Apr 5, 2012·Journal of Visualized Experiments : JoVE·Atefeh GhoghaPamela J Lein
Jul 19, 2005·Annual Review of Neuroscience·Natalia O Glebova, David D Ginty
Mar 27, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Curtis D LeeEllis Meng
Feb 12, 2009·Journal of Neurochemistry·Woo-Yang KimPamela J Lein
Jan 31, 2006·Journal of Neurochemistry·Sokhon PinMichael M Wang
Oct 22, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Jonathan PopkoLorene M Lanier
Mar 29, 2000·Molecular and Cellular Neurosciences·D Tang, D J Goldberg
Oct 31, 2014·Molecular Biology of the Cell·Olga I KahnPeter W Baas
Dec 15, 2015·Developmental Neurobiology·Lauren A CourterPamela J Lein
Aug 1, 1995·Medical Hypotheses·T H JobeM M Gaviria
Jan 17, 2002·Journal of Neurobiology·Francisco F de-Miguel, Javier Vargas
Jan 25, 2002·The Journal of Comparative Neurology·Francisco F De-MiguelCarolina Escamilla
Feb 1, 1997·Journal of Neurobiology·S K PowellM E Hatten

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