Laminin and collagen IV inclusion in immunoisolating microcapsules reduces cytokine-mediated cell death in human pancreatic islets

Journal of Tissue Engineering and Regenerative Medicine
L Alberto LlacuaPaul de Vos

Abstract

Extracellular matrix (ECM) molecules have several functions in pancreatic islets, including provision of mechanical support and prevention of cytotoxicity during inflammation. During islet isolation, ECM connections are damaged, and are not restored after encapsulation and transplantation. Inclusion of specific combinations of collagen type IV and laminins in immunoisolating capsules can enhance survival of pancreatic islets. Here we investigated whether ECM can also enhance survival and lower susceptibility of human islets to cytokine-mediated cytotoxicity. To this end, human islets were encapsulated in alginate with collagen IV and either RGD, LRE or PDSGR, i.e. laminin sequences. Islets in capsules without ECM served as control. The encapsulated islets were exposed to IL-1β, IFN-γ and TNF-α for 24 and 72 h. All combinations of ECM improved the islet cell survival, and reduced necrosis and apoptosis after cytokine exposure (P < 0.01). Collagen IV-RGD and collagen IV-LRE reduced danger-associated molecular patterns (DAMPs) release from islets (P < 0.05). Moreover, collagen IV-RGD and collagen IV-PDSGR, but not collagen IV-LRE, reduced NO release from encapsulated human islets (P < 0.05). This reduction correlated with a higher...Continue Reading

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Citations

Oct 20, 2017·Tissue Engineering. Part B, Reviews·Haitao ZhuGuoqiang Chen
Jul 28, 2019·Journal of Tissue Engineering and Regenerative Medicine·Camila Leal-LopesMari Cleide Sogayar
Feb 28, 2020·International Journal of Molecular Sciences·Shuxian HuPaul de Vos
Oct 4, 2018·Current Diabetes Reports·Antonio Citro, Harald C Ott
Jun 20, 2019·Frontiers in Bioengineering and Biotechnology·Shuixan Hu, Paul de Vos
Apr 19, 2020·Stem Cell Research & Therapy·Orianne VillardAnne Wojtusciszyn
Jan 24, 2021·Advanced Drug Delivery Reviews·Bhushan N KharbikarTejal A Desai
Jan 6, 2021·Tissue Engineering. Part B, Reviews·Rei KuwabaraPaul de Vos
Apr 16, 2021·Metabolism: Clinical and Experimental·Anna GoddiRonald N Cohen
Jun 12, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tania B Lopez-MendezRosa Maria Hernandez
Dec 11, 2020·ACS Biomaterials Science & Engineering·Alisa M WhiteXiaoming He

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