Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells

Nature Communications
Takamichi MiyazakiEihachiro Kawase


Human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have the potential to provide an infinite source of tissues for regenerative medicine. Although defined xeno-free media have been developed, culture conditions for reliable propagation of hESCs still require considerable improvement. Here we show that recombinant E8 fragments of laminin isoforms (LM-E8s), which are the minimum fragments conferring integrin-binding activity, promote greater adhesion of hESCs and hiPSCs than do Matrigel and intact laminin isoforms. Furthermore, LM-E8s sustain long-term self-renewal of hESCs and hiPSCs in defined xeno-free media with dissociated cell passaging. We successfully maintained three hESC and two hiPSC lines on LM-E8s in three defined media for 10 passages. hESCs maintained high level expression of pluripotency markers, had a normal karyotype after 30 passages and could differentiate into all three germ layers. This culture system allows robust proliferation of hESCs and hiPSCs for therapeutic applications.


Aug 1, 1988·Cell Structure and Function·T YatohgoM Hayashi
Nov 6, 1998·Science·J A ThomsonJ M Jones
May 16, 2000·The Journal of Biological Chemistry·Jarkko KortesmaaKarl Tryggvason
Mar 12, 2002·The Journal of Biological Chemistry·Sergei P SmirnovPeter D Yurchenco
Oct 3, 2002·Journal of Biochemistry·Yoshinobu KariyaKaoru Miyazaki
Dec 9, 2003·Nature Biotechnology·Jonathan S DraperPeter W Andrews
Jan 1, 2004·The Journal of Biological Chemistry·Hiroyuki IdoKiyotoshi Sekiguchi
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Jeffrey H Miner, Peter D Yurchenco
Jan 8, 2005·Nature Biotechnology·Maisam MitalipovaSteven L Stice
Jan 3, 2006·Nature Biotechnology·Tenneille E LudwigJames A Thomson
Jan 31, 2006·Nature Biotechnology·April PylePeter Donovan
Apr 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jean LuMichael Snyder
May 19, 2006·Biochemical and Biophysical Research Communications·Hirofumi SuemoriNorio Nakatsuji
Jun 7, 2006·Biochemical and Biophysical Research Communications·Yanxia LiuHongkui Deng
May 29, 2007·Nature Biotechnology·Kiichi WatanabeYoshiki Sasai
Oct 19, 2007·Current Opinion in Cell Biology·Junichi Takagi
Aug 5, 2008·Biochemical and Biophysical Research Communications·Takamichi MiyazakiHirofumi Suemori
Jan 17, 2009·The Journal of Biological Chemistry·Yukimasa TaniguchiKiyotoshi Sekiguchi
Apr 29, 2009·Journal of Cellular and Molecular Medicine·Sanna VuoristoTimo Otonkoski
Nov 26, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ying MengKevin E Healy
Feb 27, 2010·In Vitro Cellular & Developmental Biology. Animal·International Stem Cell Initiative ConsortiumHirofumi Suemori
Apr 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yue XuSheng Ding
Jun 1, 2010·Nature Biotechnology·Luis G Villa-DiazGary D Smith
Jun 1, 2010·Nature Biotechnology·Sergey RodinKarl Tryggvason
Nov 16, 2010·Nature Methods·Joseph R KlimLaura L Kiessling
Apr 12, 2011·Nature Methods·Guokai ChenJames A Thomson
Oct 11, 2011·Nature Methods·Douglas H PhanstielJoshua J Coon
Nov 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Krishanu SahaRudolf Jaenisch


May 23, 2014·Nature Materials·Adam D CelizMorgan R Alexander
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Christine-Maria HorejsMolly M Stevens
Jun 16, 2014·Nature Methods·Paul W BurridgeJoseph C Wu
Dec 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Todd C McDevitt
Jun 3, 2014·Expert Opinion on Biological Therapy·Anastasia G EfthymiouManfred Boehm
Jul 4, 2015·Annals of Medicine·Oscar E SimonsonSergey Rodin
Oct 16, 2015·FEBS Letters·Ingo RiedererWilson Savino
Feb 18, 2016·Nature Reviews. Molecular Cell Biology·Kazutoshi Takahashi, Shinya Yamanaka
Nov 21, 2013·Genesis : the Journal of Genetics and Development·Takamichi MiyazakiHirofumi Suemori
Oct 3, 2015·Cellular and Molecular Life Sciences : CMLS·Siqin WuAnna Rising
Sep 30, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mari Ohnuki, Kazutoshi Takahashi
Aug 4, 2015·Stem Cell Reports·Alex LaperleKristyn S Masters
Jan 28, 2015·Journal of Bioscience and Bioengineering·Shou-Hui DuShu Wang
Apr 19, 2015·Reproductive Biology and Endocrinology : RB&E·Nina DesaiArsela Gishto
Feb 28, 2015·Nucleic Acids Research·Tomonori NakamuraMitinori Saitou
Apr 23, 2016·Biochemical and Biophysical Research Communications·Kazuo TakayamaHiroyuki Mizuguchi
Apr 2, 2013·Biochemical and Biophysical Research Communications·Hideaki KumagaiEihachiro Kawase
Apr 26, 2016·Journal of Tissue Engineering and Regenerative Medicine·Yu Qiang SohJodhbir S Mehta
Aug 6, 2016·Seminars in Cancer Biology·Yan QinFrédéric Hollande
Sep 8, 2016·International Journal of Hematology·Yoshinori SugimineMegumu Saito
Sep 18, 2015·Stem Cells Translational Medicine·Takuji KawamuraYoshiki Sawa
Feb 19, 2016·Scientific Reports·Katsuhisa MatsuuraTeruo Okano
Jan 1, 2013·Cell Regeneration·Jack W LambsheadAndrew L Laslett
Oct 16, 2015·Journal of Dental Research·G YuH Egusa
Nov 28, 2017·Current Stem Cell Reports·Loriana Vitillo, Susan J Kimber
Jun 14, 2018·Physical Biology·L E WadkinN G Parker
Jul 3, 2018·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Masahiro Tomita
Oct 18, 2018·Physiological Reviews·Peter KaragiannisKenji Osafune
Jul 14, 2016·Nature Communications·Sara Pijuan-GalitoCecilia Annerén
Oct 24, 2018·Clinical Hemorheology and Microcirculation·Yan NieAndreas Lendlein
Mar 14, 2019·Advanced Materials·Christopher J HillPatricia Murray
Nov 5, 2013·Physiology·Andriy YabluchanskiyMerry L Lindsey
Sep 10, 2019·Inflammation and Regeneration·Masafumi UmekageNaoko Takasu
Oct 7, 2015·Current Protocols in Human Genetics·Paul W BurridgeJoseph C Wu
Feb 4, 2016·Current Protocols in Stem Cell Biology·Christian UngerOuti Hovatta
Nov 23, 2019·Journal of Peptide Science : an Official Publication of the European Peptide Society·Jun KumaiMotoyoshi Nomizu
Sep 8, 2017·Advanced Healthcare Materials·Tatsuya OsakiRoger D Kamm
Nov 27, 2019·In Vitro Cellular & Developmental Biology. Animal·Atsuko HamadaTetsuji Okamoto
Dec 2, 2017·Biotechnology Journal·Ahmed Abdal DayemSsang-Goo Cho
May 17, 2019·Essays in Biochemistry·Erhard Hohenester
Nov 25, 2019·Stem Cell Reviews and Reports·Gele LiuRichard G Fessler
Nov 15, 2019·Biology of Reproduction·Yoshitake SakaiMitinori Saitou
Jul 8, 2020·Scientific Reports·Naresh PolisettiUrsula Schlötzer-Schrehardt
Oct 11, 2014·The Journal of Biological Chemistry·Sara Pijuan-GalitoCecilia Annerén
Sep 23, 2016·Stem Cells International·Yohei Hayashi, Miho Kusuda Furue
Nov 27, 2019·World Journal of Stem Cells·Weiwei LiuGuokai Chen
Jan 14, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·J LiY Chen
Jul 13, 2017·Scientific Reports·Naresh PolisettiUrsula Schlötzer-Schrehardt
Feb 9, 2020·Nature Communications·Sissy E WamaithaKathy K Niakan
Mar 21, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Binata JoddarYoshihiro Ito
May 23, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Louise HagbardTherése Kallur
Nov 27, 2020·Frontiers in Bioengineering and Biotechnology·Andreas ElanzewOliver Brüstle
Dec 29, 2020·Advanced Healthcare Materials·Aishah NasirChris Denning
Apr 14, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Kohei KasaiShogo Miyata
Dec 4, 2019·Biomacromolecules·Daniela BarrosAna P Pêgo
Dec 29, 2020·Acta Ophthalmologica·Daniele SpinozziSilke Oellerich
Dec 17, 2020·Frontiers in Bioengineering and Biotechnology·Suman C NathDerrick E Rancourt
Dec 13, 2019·Journal of Bioscience and Bioengineering·Akihiro HashidaMasahiro Kino-Oka
Sep 11, 2018·Stem Cell Reports·Takahisa MatsuzakiTakanori Takebe
Aug 1, 2018·Biochemical and Biophysical Research Communications·Yusuke MatsudaKeiji Naruse
Apr 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Hayato SuzukiShogo Miyata
May 1, 2021·Diagnostics·Masataka YokodeYuzo Kodama
May 20, 2021·Molecular Reproduction and Development·Kazuto KimuraShingo Hatoya
May 26, 2021·PLoS Genetics·Kazutoshi TakahashiShinya Yamanaka
May 4, 2021·Ahmed K.M.A. AhmedToke Jost Isaksen

Methods Mentioned

enzymatic dissociation

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.