Human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have the potential to provide an infinite source of tissues for regenerative medicine. Although defined xeno-free media have been developed, culture conditions for reliable propagation of hESCs still require considerable improvement. Here we show that recombinant E8 fragments of laminin isoforms (LM-E8s), which are the minimum fragments conferring integrin-binding activity, promote greater adhesion of hESCs and hiPSCs than do Matrigel and intact laminin isoforms. Furthermore, LM-E8s sustain long-term self-renewal of hESCs and hiPSCs in defined xeno-free media with dissociated cell passaging. We successfully maintained three hESC and two hiPSC lines on LM-E8s in three defined media for 10 passages. hESCs maintained high level expression of pluripotency markers, had a normal karyotype after 30 passages and could differentiate into all three germ layers. This culture system allows robust proliferation of hESCs and hiPSCs for therapeutic applications.
Cell adhesion, spreading and neurite stimulation by laminin fragment E8 depends on maintenance of secondary and tertiary structure in its rod and globular domain
Failure to synthesize the T cell CD3-zeta chain: structure and function of a partial T cell receptor complex
Recombinant laminin-8 (alpha(4)beta(1)gamma(1)). Production, purification,and interactions with integrins
Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture
Recombinant human laminin-10 (alpha5beta1gamma1). Production, purification, and migration-promoting activity on vascular endothelial cells
Molecular dissection of the alpha-dystroglycan- and integrin-binding sites within the globular domain of human laminin-10
Ligand-binding specificities of laminin-binding integrins: a comprehensive survey of laminin-integrin interactions using recombinant alpha3beta1, alpha6beta1, alpha7beta1 and alpha6beta4 integrins
Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage
A novel chemical-defined medium with bFGF and N2B27 supplements supports undifferentiated growth in human embryonic stem cells
The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma chains in integrin binding by laminins
Recombinant vitronectin is a functionally defined substrate that supports human embryonic stem cell self-renewal via alphavbeta5 integrin
Recombinant human laminin isoforms can support the undifferentiated growth of human embryonic stem cells
Comparison of defined culture systems for feeder cell free propagation of human embryonic stem cells
Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules
Synthetic peptide-acrylate surfaces for long-term self-renewal and cardiomyocyte differentiation of human embryonic stem cells
Individual cell movement, asymmetric colony expansion, rho-associated kinase, and E-cadherin impact the clonogenicity of human embryonic stem cells
High-content screening of feeder-free human embryonic stem cells to identify pro-survival small molecules
An optimized small molecule inhibitor cocktail supports long-term maintenance of human embryonic stem cells
E-cadherin is crucial for embryonic stem cell pluripotency and can replace OCT4 during somatic cell reprogramming
Surface-engineered substrates for improved human pluripotent stem cell culture under fully defined conditions
Development of a simple, repeatable, and cost-effective extracellular matrix for long-term xeno-free and feeder-free self-renewal of human pluripotent stem cells.
Comparative proteomic analysis of supportive and unsupportive extracellular matrix substrates for human embryonic stem cell maintenance.
A novel feeder-free culture system for human pluripotent stem cell culture and induced pluripotent stem cell derivation
Generation of human induced pluripotent stem (Ips) cells in serum- and feeder-free defined culture and TGF-Β1 regulation of pluripotency
Toward the development of bioengineered human three-dimensional vascularized cardiac tissue using cell sheet technology
Biologically-active laminin-111 fragment that modulates the epithelial-to-mesenchymal transition in embryonic stem cells
Cationic surface charge combined with either vitronectin or laminin dictates the evolution of human embryonic stem cells/microcarrier aggregates and cell growth in agitated cultures
Inhibition of apoptosis in human induced pluripotent stem cells during expansion in a defined culture using angiopoietin-1 derived peptide QHREDGS
Monolayer culturing and cloning of human pluripotent stem cells on laminin-521-based matrices under xeno-free and chemically defined conditions
Efficient and scalable expansion of human pluripotent stem cells under clinically compliant settings: a view in 2013
Self-renewal and cell lineage differentiation strategies in human embryonic stem cells and induced pluripotent stem cells
Simple and versatile synthetic polydopamine-based surface supports reprogramming of human somatic cells and long-term self-renewal of human pluripotent stem cells under defined conditions
Protein kinase A inhibitor, H89, enhances survival and clonogenicity of dissociated human embryonic stem cells through Rho-associated coiled-coil containing protein kinase (ROCK) inhibition
Cardiomyocytes Derived from MHC-Homozygous Induced Pluripotent Stem Cells Exhibit Reduced Allogeneic Immunogenicity in MHC-Matched Non-human Primates
Bio-inspired oligovitronectin-grafted surface for enhanced self-renewal and long-term maintenance of human pluripotent stem cells under feeder-free conditions
Efficient passage of human pluripotent stem cells on spider silk matrices under xeno-free conditions
Cultured Cells from the Human Oocyte Cumulus Niche Are Efficient Feeders to Propagate Pluripotent Stem Cells
Down-regulation of cell adhesion via rho-associated protein kinase (ROCK) pathway promotes tumor cell migration on laminin-511
Influence of substrate composition on human embryonic stem cell differentiation and extracellular matrix production in embryoid bodies
Physical, Spatial, and Molecular Aspects of Extracellular Matrix of In Vivo Niches and Artificial Scaffolds Relevant to Stem Cells Research
Spider silk for xeno-free long-term self-renewal and differentiation of human pluripotent stem cells
Technological progress and challenges towards cGMP manufacturing of human pluripotent stem cells based therapeutic products for allogeneic and autologous cell therapies
Selective In Vitro Propagation of Nephron Progenitors Derived from Embryos and Pluripotent Stem Cells.
Human embryonic stem cell cultivation: historical perspective and evolution of xeno-free culture systems
Isolation of human induced pluripotent stem cell-derived dopaminergic progenitors by cell sorting for successful transplantation
Integrating mechanical and biological control of cell proliferation through bioinspired multieffector materials
Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells
A defined xeno-free and feeder-free culture system for the derivation, expansion and direct differentiation of transgene-free patient-specific induced pluripotent stem cells
Controlled Growth and the Maintenance of Human Pluripotent Stem Cells by Cultivation with Defined Medium on Extracellular Matrix-Coated Micropatterned Dishes
A novel in vitro method for detecting undifferentiated human pluripotent stem cells as impurities in cell therapy products using a highly efficient culture system
Construction of a Defined Biomimetic Matrix for Long-Term Maintenance of Mouse Induced Pluripotent Stem Cells
Protein kinase A inhibitor, H89, significantly enhances survival rate of dissociated human embryonic stem cells following cryopreservation
Integrin-Associated Focal Adhesion Kinase Protects Human Embryonic Stem Cells from Apoptosis, Detachment, and Differentiation
The Sal-like 4 - integrin α6β1 network promotes cell migration for metastasis via activation of focal adhesion dynamics in basal-like breast cancer cells
A portable platform for stepwise hematopoiesis from human pluripotent stem cells within PET-reinforced collagen sponges
Structural Changes in N-Glycans on Induced Pluripotent Stem Cells Differentiating Toward Cardiomyocytes
Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs
Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells
A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells
Human embryonic and induced pluripotent stem cells maintain phenotype but alter their metabolism after exposure to ROCK inhibitor
Efficient Adhesion Culture of Human Pluripotent Stem Cells Using Laminin Fragments in an Uncoated Manner
Natural and Synthetic Materials for Self-Renewal, Long-Term Maintenance, and Differentiation of Induced Pluripotent Stem Cells
Scaling-Up Techniques for the Nanofabrication of Cell Culture Substrates via Two-Photon Polymerization for Industrial-Scale Expansion of Stem Cells
Designing well-defined photopolymerized synthetic matrices for three-dimensional culture and differentiation of induced pluripotent stem cells
Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment
Generation of safe and therapeutically effective human induced pluripotent stem cell-derived hepatocyte-like cells for regenerative medicine
Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture
Xeno-free and feeder-free culture and differentiation of human embryonic stem cells on recombinant vitronectin-grafted hydrogels
Induction of integration-free human-induced pluripotent stem cells under serum- and feeder-free conditions
Preconditioning the Initial State of Feeder-free Human Pluripotent Stem Cells Promotes Self-formation of Three-dimensional Retinal Tissue
Characterization of dystroglycan binding in adhesion of human induced pluripotent stem cells to laminin-511 E8 fragment
Efficient and scalable culture of single dissociated human pluripotent stem cells using recombinant E8 fragments of human laminin isoforms
Laminin 521 Stabilizes the Pluripotency Expression Pattern of Human Embryonic Stem Cells Initially Derived on Feeder Cells
CD200 facilitates the isolation of corneal epithelial cells derived from human pluripotent stem cells
Serum Inter-α-inhibitor activates the Yes tyrosine kinase and YAP/TEAD transcriptional complex in mouse embryonic stem cells.
Poly-l-Lactic Acid Nanofiber-Polyamidoamine Hydrogel Composites: Preparation, Properties, and Preliminary Evaluation as Scaffolds for Human Pluripotent Stem Cell Culturing
Culture substrates made of elastomeric micro-tripod arrays for long-term expansion of human pluripotent stem cells
Chemically defined human vascular laminins for biologically relevant culture of hiPSC-derived brain microvascular endothelial cells.
Current strategies to generate mature human induced pluripotent stem cells derived cholangiocytes and future applications
Laminin-511 and -521-based matrices for efficient ex vivo-expansion of human limbal epithelial progenitor cells
A Simple and Robust Method for Culturing Human-Induced Pluripotent Stem Cells in an Undifferentiated State Using Botulinum Hemagglutinin
Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip
Chemically defined and growth-factor-free culture system for the expansion and derivation of human pluripotent stem cells
MHC-mismatched Allotransplantation of Induced Pluripotent Stem Cell-Derived Cardiomyocyte Sheets to Improve Cardiac Function in A Primate Ischemic Cardiomyopathy Model
Robust and highly efficient hiPSC generation from patient non-mobilized peripheral blood-derived CD34+ cells using the auto-erasable Sendai virus vector
Chemically fixed autologous feeder cell-derived niche for human induced pluripotent stem cell culture
Self-Renewal Capability of Hepatocytic Parental Progenitor Cells Derived From Adult Rat Liver Is Maintained Long Term When Cultured on Laminin 111 in Serum-Free Medium
Changes of plasmalogen phospholipid levels during differentiation of induced pluripotent stem cells 409B2 to endothelial phenotype cells
A Novel Fluorescent Reporter System Identifies Laminin-511/521 as Potent Regulators of Cardiomyocyte Maturation.
Decrease of laminin-511 in the basement membrane due to photoaging reduces epidermal stem/progenitor cells.
Voltage-gated ion channels mediate the electrotaxis of glioblastoma cells in a hybrid PMMA/PDMS microdevice
The StemCellFactory: A Modular System Integration for Automated Generation and Expansion of Human Induced Pluripotent Stem Cells
Discovery of a Novel Polymer for Xeno-Free, Long-Term Culture of Human Pluripotent Stem Cell Expansion.
Cost-effective culture of human induced pluripotent stem cells using UV/ozone-modified culture plastics with reduction of cell-adhesive matrix coating
Chitosan 3D cell culture system promotes naïve-like features of human induced pluripotent stem cells: A novel tool to sustain pluripotency and facilitate differentiation.
Critical Roles of Translation Initiation and RNA Uridylation in Endogenous Retroviral Expression and Neural Differentiation in Pluripotent Stem Cells.
Endometrial regeneration with endometrial epithelium: homologous orchestration with endometrial stroma as a feeder.
The immobilization of fibronectin- and fibroblast growth factor 2-derived peptides on a culture plate supports the attachment and proliferation of human pluripotent stem cells.
Generation of human induced pluripotent stem cell-derived liver buds with chemically defined and animal origin-free media.
Kinetics on aggregate behaviors of human induced pluripotent stem cells in static suspension and rotating flow cultures
Adequate taylor couette flow-mediated shear stress is useful for dissociating human iPS cell-derived cell aggregates
Human gingival fibroblast feeder cells promote maturation of induced pluripotent stem cells into cardiomyocytes
Extracellular laminin regulates hematopoietic potential of pluripotent stem cells through integrin β1-ILK-β-catenin-JUN axis.
UV/ozone surface modification combined with atmospheric pressure plasma irradiation for cell culture plastics to improve pluripotent stem cell culture.
Defining optimal enzyme and matrix combination for replating of human induced pluripotent stem cell-derived cardiomyocytes at different levels of maturity.
Canine induced pluripotent stem cell maintenance under feeder-free and chemically-defined conditions.
Adhesion Molecules in Health and Disease
Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.
Allogenic & Autologous Therapies
Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.