PMID: 2503413May 1, 1989Paper

Laminin induces formation of neurite-like processes and potentiates prolactin secretion by GH3 rat pituitary cells

Differentiation; Research in Biological Diversity
N Brunet-de CarvalhoA Tixier-Vidal

Abstract

Tumor-derived GH3 rat pituitary cell lines are widely utilized to study mechanisms of prolactin secretion and responsiveness to secretagogues. These cells served here as a model with which to study relationships between shape and function. When GH3 cells were routinely grown in serum-supplemented medium, they exhibited the polygonal phenotype of epithelial cells, with scarce secretory granules. In contrast, when seeded in a serum-free medium, they attached loosely and contained more secretory granules. In both cases, they released prolactin in a nonpolarized manner. We show in the present work that laminin extracted from Englebreth-Holm-Swarm (EHS) tumors was a potent attachment and spreading factor for GH3/B6 cells seeded in serum-free medium. Moreover, it induced the formation of neurite-like processes, which were increased in number and length by chronic treatment with a specific secretagogue, thyroliberin (TRH). These changes in cell shape were correlated with a potentiation of prolactin secretion, both basal and TRH-stimulated. Furthermore, using immunocytochemistry and electron microscopy, we revealed--at the dilated tip of processes--an accumulation not only of prolactin, but also of synaptophysin, a vesicle membrane mar...Continue Reading

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Citations

May 1, 1992·Microscopy Research and Technique·K WatanabeH Iwasaki
Sep 1, 1991·Molecular and Cellular Endocrinology·J N LaverrièreD Gourdji

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