Laminin receptor mediates anti-inflammatory and anti-thrombogenic effects of pigment epithelium-derived factor in myeloma cells

Biochemical and Biophysical Research Communications
Takanori MatsuiSho-ichi Yamagishi

Abstract

Pigment epithelium-derived factor (PEDF) has anti-inflammatory and anti-thrombogenic properties both in cell culture and animal models. Although adipose triglyceride lipase (ATGL) and laminin receptor (LR) are two putative receptors for PEDF, which receptor mainly mediates the beneficial effects of PEDF is largely unknown. In this study, we addressed the issue. siRNA raised against LR (siLR) and siATGL transfection dramatically decreased LR and ATGL levels in human cultured myeloma cells, respectively. Ten nM PEDF significantly reduced vascular endothelial growth factor (VEGF), monocyte chemoattractant protein-1 (MCP-1), intercellular cell adhesion molecule-1 (ICAM-1) and plasminogen activator inhibitor-1 (PAI-1) mRNA levels in siCon- or siATGL-transfected myeloma cells, whereas PEDF increased rather than decreased these gene expressions in siLR-transfected cells. Neutralizing antibody directed against LR (LR-Ab) or LR antagonist actually bound to LR and reduced mRNA levels of VEGF, MCP-1, ICAM-1 and PAI-1 in myeloma cells. Further, pre-treatment of LR-Ab or LR antagonist suppressed the binding of PEDF to LR and resultantly blocked the effects of PEDF in myeloma cells. In addition, high concentration of LR agonist mimicked the ...Continue Reading

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Citations

Dec 16, 2016·International Journal of Molecular Sciences·Zhongxin ZhouZhongming Zhang
Mar 21, 2019·Current Pharmaceutical Design·Sho-Ichi YamagishiMinoru Yagi
Oct 10, 2015·Molecular Cancer Therapeutics·Keren GoldbergIrit Ben-Aharon
Sep 15, 2019·Experimental Eye Research·Nader SheibaniJack Henkin
Aug 2, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yu ZhangXianjie Ma

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