Laminin stimulates rapid epithelial restitution of rabbit duodenal mucosa in vitro

Scandinavian Journal of Gastroenterology
M RieglerE Wenzl

Abstract

This study investigated the effect of the basal lamina constituents fibronectin, collagen IV, and laminin on epithelial restitution of rabbit duodenum in vitro. Rabbit duodenal mucosal sheets were mounted in Ussing chambers, luminally exposed to 10 mM HCI for 10 min, and incubated with buffer or luminal buffer containing 25-100 micrograms/ml of collagen IV, fibronectin, laminin, or polyclonal antisera directed against these proteins (diluted 1:50-1:20) for 3 h. Resistance was calculated from potential difference and short-circuit current. Mucosal damage was assessed by morphometry on hematoxylin- and eosin-stained sections. Acid exposure caused a 40% drop in resistance (119 +/- 5 versus 71 +/- 5 Ohm.cm2 before versus after injury; P < 0.05, n = 6) and mucosal damage of 58 +/- 4% (n = 6). Three hours after injury resistance was 102 +/- 6, 117 +/- 4, and 48 +/- 5 Ohm.cm2 in the control, laminin, and anti-laminin groups, respectively. Furthermore, 36 +/- 2%, 16 +/- 2%, and 64 +/- 5% of the mucosa was damaged in the control, laminin, and anti-laminin groups, respectively, 3 h after injury (P < 0.05 versus controls). Laminin promoted epithelial wound closure by stimulation of enterocyte migration, which was inhibited by anti-laminin...Continue Reading

References

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Citations

Dec 28, 2007·Pharmaceutical Research·Yogeeta NarkarJoseph R Robinson
Jan 15, 2000·Scandinavian Journal of Gastroenterology·M RieglerC Pothoulakis
Apr 13, 2007·Physiological Reviews·Anthony T BlikslagerJack Odle
Jun 24, 1998·Biochemical and Biophysical Research Communications·D YuR Petryshyn

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