LAMP-PCR detection of ochratoxigenic Aspergillus species collected from peanut kernel

Genetics and Molecular Research : GMR
H M Al-Sheikh

Abstract

Over the last decade, ochratoxin A (OTA) has been widely described and is ubiquitous in several agricultural products. Ochratoxins represent the second-most important mycotoxin group after aflatoxins. A total of 34 samples were surveyed from 3 locations, including Mecca, Madina, and Riyadh, Saudi Arabia, during 2012. Fungal contamination frequency was determined for surface-sterilized peanut seeds, which were seeded onto malt extract agar media. Aspergillus niger (35%), Aspergillus ochraceus (30%), and Aspergillus carbonarius (25%) were the most frequently observed Aspergillius species, while Aspergillus flavus and Aspergillus phoenicis isolates were only infrequently recovered and in small numbers (10%). OTA production was evaluated on yeast extract sucrose medium, which revealed that 57% of the isolates were A. niger and 60% of A. carbonarius isolates were OTA producers; 100% belonged to A. ochraceus. Only one isolate, morphologically identified as A. carbonarius, and 3 A. niger isolates unstably produced OTA. A polymerase chain reaction (PCR)-based identification and detection assay was used to identify A. ochraceus isolates. Using the primer sets OCRA1/OCRA2, 400-base pair PCR fragments were produced only when genomic DNA f...Continue Reading

Citations

Aug 13, 2015·Journal of Microbiological Methods·Tuba NasriMojtaba Nabili
Feb 9, 2019·Molecular Ecology Resources·Robert M W FergusonIan Colbeck
Sep 29, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Zin Mar HtunTheerapong Krajaejun

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