LAMP3 plays an oncogenic role in osteosarcoma cells partially by inhibiting TP53

Cellular & Molecular Biology Letters
Shaoxian LiuWeidong Zhang

Abstract

Osteosarcoma (OS) is a common malignant tumor that predominantly occurs in adolescents. Its most common metastasis is to the lungs. As shown in our earlier study, lysosome-associated membrane glycoprotein 3 (LAMP3) is highly upregulated in metastatic OS. However, its role in the regulation of OS cell viability and apoptosis remains unknown. We knocked down and overexpressed LAMP3 in OS cells and assessed the cell viability and apoptosis. Then, we investigated the expression of apoptosis-associated genes to identify the downstream gene(s) of LAMP3. Knockdown of LAMP3 significantly inhibited OS cell viability and promoted apoptosis. TP53, which is involved in the apoptosis pathway, was found to be highly upregulated after knockdown of LAMP3. Overexpression of LAMP3 significantly increased cell viability and abrogated apoptosis. Importantly, subsequent knockdown of TP53 partially suppressed the increased OS cell apoptosis induced by the inhibition of LAMP3, suggesting that TP53 is a key functional downstream gene of LAMP3. Our findings suggest that LAMP3 promotes OS cell viability and survival by regulating TP53 expression.

References

Feb 3, 2012·Journal of Comparative Pathology·T S Osborne, C Khanna
Sep 18, 2012·Pharmacology & Therapeutics·Jonathan GillRichard Gorlick
May 29, 2014·Database : the Journal of Biological Databases and Curation·Kathrin PoosEberhard Korsching
Sep 10, 2014·International Journal of Cancer. Journal International Du Cancer·Wenqing TangLing Dong
Nov 18, 2014·Oncotarget·Alessandra BisioAlberto Inga
Aug 13, 2015·International Journal of Molecular Sciences·Xiaoyu LiaoWeihua Jia
Mar 22, 2016·Expert Review of Anticancer Therapy·Cristina Meazza, Paolo Scanagatta
Apr 9, 2016·International Journal of Molecular Sciences·Atik Badshah ShaikhGe Zhang
Apr 14, 2016·Future Oncology·Vadim V Maximov, Rami I Aqeilan
Jun 7, 2016·Current Problems in Pediatric and Adolescent Health Care·Twana M JacksonLinda Granowetter
Jul 2, 2016·Stem Cells International·Ander AbarrategiRene Rodriguez
Oct 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jiao YangJin Yang
Oct 21, 2016·Kidney & Blood Pressure Research·You KeHua-Jun Jiang

❮ Previous
Next ❯

Citations

Apr 22, 2020·The Journal of Biological Chemistry·Thomas D BurtonChristopher G Proud
Aug 9, 2020·Cancer Management and Research·Peng HanFan Li
Feb 1, 2021·The Journal of Biological Chemistry·Thomas D BurtonChristopher G Proud

❮ Previous
Next ❯

Methods Mentioned

BETA
surgical resection
transfection
flow cytometry

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.