LANCL1 binds abscisic acid and stimulates glucose transport and mitochondrial respiration in muscle cells via the AMPK/PGC-1α/Sirt1 pathway.

Molecular Metabolism
Sonia SpinelliLaura Sturla

Abstract

Abscisic acid (ABA) is a plant hormone also present and active in animals. In mammals, ABA regulates blood glucose levels by stimulating insulin-independent glucose uptake and metabolism in adipocytes and myocytes through its receptor LANCL2. The objective of this study was to investigate whether another member of the LANCL protein family, LANCL1, also behaves as an ABA receptor and, if so, which functional effects are mediated by LANCL1. ABA binding to human recombinant LANCL1 was explored by equilibrium-binding experiments with [3H]ABA, circular dichroism, and surface plasmon resonance. Rat L6 myoblasts overexpressing either LANCL1 or LANCL2, or silenced for the expression of both proteins, were used to investigate the basal and ABA-stimulated transport of a fluorescent glucose analog (NBDG) and the signaling pathway downstream of the LANCL proteins using Western blot and qPCR analysis. Finally, glucose tolerance and sensitivity to ABA were compared in LANCL2-/- and wild-type (WT) siblings. Human recombinant LANCL1 binds ABA with a Kd between 1 and 10 μM, depending on the assay (i.e., in a concentration range that lies between the low and high-affinity ABA binding sites of LANCL2). In L6 myoblasts, LANCL1 and LANCL2 similarly...Continue Reading

References

Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M T Le Page-DegivryM Lazdunski
Jun 9, 1997·Biochemical and Biophysical Research Communications·A Vidal-PuigB B Lowell
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kara HunterLinda S Wicker
Aug 12, 2009·The Journal of Biological Chemistry·Laura SturlaElena Zocchi
Sep 22, 2010·Journal of Cellular Physiology·Jung Ok LeeHyeon Soo Kim
Nov 1, 2011·Biochemical and Biophysical Research Communications·Laura SturlaElena Zocchi
Nov 15, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Santina BruzzoneElena Zocchi
Jan 18, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Heather F CrouseSwarna Basu
Oct 3, 2014·Molecular Biology of the Cell·Min ZengJie Chen
Apr 8, 2015·The Journal of Biological Chemistry·Tiziana VigliaroloElena Zocchi
Aug 6, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mirko MagnoneElena Zocchi
May 26, 2016·Scientific Reports·Chiara FresiaElena Zocchi
Jan 21, 2017·Scientific Reports·Chang HeWilfred A van der Donk
Jul 21, 2017·Cell Reports·Lu YaoYongsheng Chang
Aug 3, 2017·American Journal of Physiology. Endocrinology and Metabolism·Xiaona CuiYongsheng Chang
Feb 9, 2018·The International Journal of Biochemistry & Cell Biology·Elena CicheroPaola Fossa
Jun 9, 2018·Journal of Lipid Research·Debapriya DuttaWilfred A van der Donk
Apr 24, 2019·Diabetes·Rasmus KjøbstedJørgen F P Wojtaszewski
Oct 19, 2019·Journal of Molecular Endocrinology·Lena EspelageAlexandra Chadt
Jun 10, 2020·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Jens Frey Halling, Henriette Pilegaard
Nov 26, 2020·International Journal of Environmental Research and Public Health·Jonathan J Petrocelli, Micah J Drummond

❮ Previous
Next ❯

Software Mentioned

GraphPad Prism
Biacore T200
Biacore Evaluation
iQ5 Optical System
Beacon Designer

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.