Landscape of T Follicular Helper Cell Dynamics in Human Germinal Centers.

The Journal of Immunology : Official Journal of the American Association of Immunologists
Emmanuel DonnadieuMartin-Leo Hansmann

Abstract

T follicular helper (Tfh) cells play a very important role in mounting a humoral response. Studies conducted in mouse models have revealed with good kinetic and spatial resolution the dynamics of these cells in germinal centers (GC) and their cross-talk with B cells upon an immune response. However, whether a similar migratory behavior is performed by human Tfh cells is unclear, as technology to track them in situ has been lacking. In this study, we combined traditional immunohistochemistry and real-time fluorescent imaging approaches on fresh human adenoid slices to provide static and dynamic information on Tfh cells. Our data indicate that GC light zones are composed of two distinct areas in terms of Tfh cell distribution and migration. In the outer GC light zones, Tfh cells migrate actively and with a high ability to form dynamic clusters showing intense and rapid reorganization. In these outer regions, Tfh cells demonstrate multiple interactions between each other. Conversely, in central regions of GC light zones, Tfh cells are much more static, forming long-lasting conjugates. These findings reveal for the first time, to our knowledge, the dynamic behavior whereby Tfh cells migrate in human GC and highlight the heterogenei...Continue Reading

References

May 1, 1993·European Journal of Immunology·D L HardieI C MacLennan
Dec 23, 2006·Science·Christopher D C AllenJason G Cyster
May 9, 2007·The Journal of Experimental Medicine·François Asperti-BoursinEmmanuel Donnadieu
Feb 2, 2012·The Journal of Clinical Investigation·Hélène SalmonEmmanuel Donnadieu
Mar 5, 2013·Advances in Experimental Medicine and Biology·Nathalie Schmitt, Hideki Ueno
Jan 17, 2015·Nature Immunology·Hideki UenoCarola G Vinuesa
Feb 9, 2016·Nature Reviews. Immunology·Matthew F KrummelAudrey Gérard
Feb 26, 2016·Annual Review of Immunology·Carola G VinuesaIan C M MacLennan
Aug 31, 2016·Nature Reviews. Immunology·Hai Qi
Apr 11, 2018·Proceedings of the National Academy of Sciences of the United States of America·Elisa PeranzoniEmmanuel Donnadieu
Dec 14, 2018·Frontiers in Immunology·Monika C Brunner-Weinzierl, Christopher E Rudd
Jan 6, 2019·Methods in Molecular Biology·Emmanuel DonnadieuMartin-Leo Hansmann
Mar 16, 2019·Immunological Reviews·Wenzhi Song, Joe Craft

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