Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni

Scientific Reports
Mariana BoroniGlória R Franco

Abstract

Spliced leader dependent trans-splicing (SLTS) has been described as an important RNA regulatory process that occurs in different organisms, including the trematode Schistosoma mansoni. We identified more than seven thousand putative SLTS sites in the parasite, comprising genes with a wide spectrum of functional classes, which underlines the SLTS as a ubiquitous mechanism in the parasite. Also, SLTS gene expression levels span several orders of magnitude, showing that SLTS frequency is not determined by the expression level of the target gene, but by the presence of particular gene features facilitating or hindering the trans-splicing mechanism. Our in-depth investigation of SLTS events demonstrates widespread alternative trans-splicing (ATS) acceptor sites occurring in different regions along the entire gene body, highlighting another important role of SLTS generating alternative RNA isoforms in the parasite, besides the polycistron resolution. Particularly for introns where SLTS directly competes for the same acceptor substrate with cis-splicing, we identified for the first time additional and important features that might determine the type of splicing. Our study substantially extends the current knowledge of RNA processing ...Continue Reading

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Citations

Sep 16, 2020·Wiley Interdisciplinary Reviews. RNA·Bingbing XuYongfeng Jin
Nov 5, 2019·Frontiers in Genetics·Sandra Grossi GavaMarina Moraes Mourão
Aug 12, 2021·Trends in Genetics : TIG·Oriana Q H ZinaniErtuğrul M Özbudak
Sep 22, 2021·G3 : Genes - Genomes - Genetics·Darrin T SchultzRichard E Green
Oct 20, 2021·Genome Biology and Evolution·Hsiao-Pei YangFay-Wei Li

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Datasets Mentioned

BETA
SRR1134198
SRR1134204

Methods Mentioned

BETA
RNA-Seq
Assay
PCR

Software Mentioned

Samtools
SLTS
Easy Promoter prediction Program ( EP3 )
TopHat2 mapper
rpsblast
WebLogo
Illumina adapter
EP3
FastQC
blastn

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