PMID: 8941667Dec 1, 1996Paper

Langerhans cells express inducible nitric oxide synthase and produce nitric oxide

The Journal of Investigative Dermatology
A A QureshiE A Lerner

Abstract

The importance of nitric oxide (NO) in mediating macrophage functions has been demonstrated, but production of this potent gas has not been examined in Langerhans cells (LC). Using murine LC purified from epidermal cell suspensions and the recently established LC-like cell line derived from newborn BALB/c epidermis (XS-52), it was shown with reverse transcriptase (RT)-PCR that inducible nitric oxide synthase (iNOS) message is present in these cells. Murine keratinocytes did not contain iNOS message. iNOS mRNA was increased in a concentration-dependent manner by lipopolysaccharide (LPS) in purified murine LC and XS-52 cells, and immunofluorescence using an antibody to iNOS revealed bright cytoplasmic staining in LPS-treated XS-52 cells. Anti-iNOS antibody brightly stained LC on human neonatal foreskin cryosections. An increase in NO production by LPS-treated XS-52 cells over 16 h, as measured by the determination of nitrite levels in culture supernatants using the Griess Reaction, was observed. Interferon-gamma (IFNgamma) did not affect NO production on its own. In the presence of LPS and IFNgamma, NO production was 3 times more than observed with LPS alone. NO production was inhibited by the NOS inhibitor L-NAME. Western blots ...Continue Reading

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Citations

Sep 29, 2001·Nature Immunology·C Bogdan
Apr 20, 2012·Free Radical Research·Vania Aparecida TerraAlessandra Lourenco Cecchini
May 16, 2000·The British Journal of Dermatology·A D OrmerodS A Shah
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Oct 9, 2002·The American Journal of Pathology·Maria Laura GiustizieriGiampiero Girolomoni

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