Apr 22, 2020

Antigenicity, stability, and reproducibility of Zika reporter virus particles for long-term applications

BioRxiv : the Preprint Server for Biology
J. C. WhitbeckBenjamin J Doranz

Abstract

The development of vaccines against flaviviruses, including Zika virus (ZIKV) and dengue virus (DENV), continues to be a major challenge, hindered by the lack of efficient and reliable methods for screening neutralizing activity of sera or antibodies. To address this need, we previously developed a plasmid-based, replication-incompetent DENV reporter virus particle (RVP) production system as an efficient and safe alternative to the Plaque Reduction Neutralization Test (PRNT). As part of the response to the 2015-2016 ZIKV outbreak, we recently developed pseudo-infectious ZIKV RVPs by modifying our DENV RVP system. The use of ZIKV RVPs as critical reagents in human clinical trials requires their further validation using stability and reproducibility metrics for large-scale applications. In the current study, we validated ZIKV RVPs using infectivity, neutralization, and enhancement assays with monoclonal antibodies (MAbs) and human ZIKV-positive patient serum. ZIKV RVPs are antigenically identical to live virus based on binding ELISA and neutralization results and are nonreplicating based on the results of plaque formation assays. We demonstrate reproducible neutralization titer data (NT50 values) across different RVP production l...Continue Reading

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Mentioned in this Paper

Positioning Attribute
Pyotraumatic Dermatitis
Speech Sound Disorders
Genes
Hot Spot
Gene Deletion Abnormality
Gene Deletion
Autistic Disorder
Speech Delay
Deletion Mutation

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