Apr 20, 2020

Decoding molecular markers and transcriptional circuitry of naive and primed states of human pluripotency

BioRxiv : the Preprint Server for Biology
A. Ghosh, Anup Som

Abstract

Pluripotent stem cells (PSCs) have been observed to occur in two distinct states - naive and primed. Both naive and primed state PSCs can give rise to tissues of all the three germ layers in vitro but differ in their potential to generate germline chimera in vivo. Understanding the molecular mechanisms that govern these two states of pluripotency in human can open up a plethora of opportunities for studying early embryonic development and in biomedical applications. In this work, we use weighted gene co-expression network (WGCN) approach to identify the key molecular makers and their interactions that define the two distinct pluripotency states. Signed-hybrid WGCN was reconstructed from transcriptomic data (RNA-seq) of naive and primed state pluripotent samples. Our analysis revealed two sets of genes that are involved in establishment and maintenance of naive (4791 genes) and primed (5066 genes) states. The naive state genes were found to be enriched for biological processes and pathways related to metabolic processes while primed state genes were associated with system development. Further, we identified the top 10% genes by intra-modular connectivity as hubs and the hub transcription factors for each group, thus providing a ...Continue Reading

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Mentioned in this Paper

Genes
Brain
Gene Mutation
Neurodevelopmental Disorders
Morphological
Persons
Autism Spectrum Disorders
Hypofunction
Bone Structure of Cranium
Domestic Abuse

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