Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity

Chemical Communications : Chem Comm
Juan A González-VeraMay C Morris

Abstract

We describe a lanthanide biosensor that responds to CDK4 kinase activity in melanoma cell extracts through a significant and dose dependent increase in luminescence, thanks to sensitization of a DOTA[Tb3+] complex incorporated into a CDK4 substrate peptide by a unique tryptophan residue in an adjacent phosphoaminoacid binding moiety.

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Citations

May 24, 2018·Chemical Communications : Chem Comm·Sarah H Hewitt, Stephen J Butler
Apr 4, 2019·Organic & Biomolecular Chemistry·Feng GaoNeal J Zondlo
Nov 15, 2020·Methods and Protocols·M Carmen Gonzalez-GarciaAngel Orte

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