Lanthanide-Dependent Methanol Dehydrogenases of XoxF4 and XoxF5 Clades Are Differentially Distributed Among Methylotrophic Bacteria and They Reveal Different Biochemical Properties

Frontiers in Microbiology
Jing HuangLudmila Chistoserdova

Abstract

Lanthanide-dependent alcohol dehydrogenases have recently emerged as environmentally important enzymes, most prominently represented in methylotrophic bacteria. The diversity of these enzymes, their environmental distribution, and their biochemistry, as well as their evolutionary relationships with their calcium-dependent counterparts remain virtually untapped. Here, we make important advances toward understanding lanthanide-dependent methylotrophy by assessing the distribution of XoxF4 and XoxF5 clades of lanthanide methanol dehydrogenases among, respectively, Methylophilaceae and non-Methylophilaceae methylotrophs, and we carry out comparative biochemical characterization of XoxF4 and XoxF5 enzymes, demonstrating differences in their properties, including catalytic efficiencies. We conclude that one subtype of the XoxF4 enzyme, XoxF4-1 is the dominant type in nature while other XoxF4 subtypes appear to be auxiliary, representatives of this clade only found in the Methylophilaceae (Betaproteobacteria). In contrast, we demonstrate that XoxF5 enzymes are widespread among Alpha-, Beta-, and Gammaproteobacteria. We purified and biochemically characterized two XoxF4 enzymes (XoxF4-1 and XoxF4-2), both from Methylotenera mobilis, an...Continue Reading

References

Sep 1, 1989·European Journal of Biochemistry·J FrankJ A Duine
Dec 1, 2000·Applied and Environmental Microbiology·A J AumanM E Lidstrom
Feb 24, 2001·Sub-cellular Biochemistry·C Anthony
May 6, 2003·Journal of Bacteriology·Ludmila ChistoserdovaMary E Lidstrom
Jul 6, 2004·Archives of Biochemistry and Biophysics·Christopher Anthony
Sep 1, 2004·Cellular and Molecular Life Sciences : CMLS·S Lim, S J Franklin
Dec 13, 2006·International Journal of Systematic and Evolutionary Microbiology·Marina G KalyuzhnayaLudmila Chistoserdova
Apr 9, 2008·Environmental Microbiology·Stephen J GiovannoniMichael S Rappé
Jul 25, 2009·International Journal of Systematic and Evolutionary Microbiology·Munusamy MadhaiyanTong-Min Sa
Oct 16, 2010·Nature Reviews. Microbiology·Brajesh K SinghDave S Reay
Jan 25, 2011·Journal of Bioscience and Bioengineering·Yoshihisa HibiKeiichi Kawai
Feb 22, 2011·Journal of Bioscience and Bioengineering·Nanung Agus FitriyantoKeiichi Kawai
Mar 30, 2011·Environmental Microbiology·Ludmila Chistoserdova
Jun 8, 2012·Standards in Genomic Sciences·Megan J HuggettMichael S Rappé
Nov 17, 2012·Nature Reviews. Microbiology·Julia A Vorholt
Mar 12, 2013·Journal of Bacteriology·Ildar MustakhimovLudmila Chistoserdova
Sep 17, 2013·Environmental Microbiology·Arjan PolHuub J M Op den Camp
May 13, 2014·Applied Microbiology and Biotechnology·Jan T KeltjensHuub J M Op den Camp
Dec 21, 2014·Applied and Environmental Microbiology·Ming L WuLaura van Niftrik
Feb 7, 2015·Genome Announcements·Tami L McTaggartLudmila Chistoserdova
Feb 24, 2015·Genome Announcements·Tami L McTaggartLudmila Chistoserdova
Mar 15, 2015·Genome Announcements·Marina G KalyuzhnayaLudmila Chistoserdova
May 7, 2015·Environmental Microbiology·Martin TaubertJ Colin Murrell
Aug 25, 2015·Applied and Environmental Microbiology·Muhammad Farhan Ul HaqueJeremy D Semrau
Dec 4, 2015·Nature·Yang BaiPaul Schulze-Lefert
Dec 30, 2015·Applied and Environmental Microbiology·Muhammad Farhan Ul HaqueJeremy D Semrau
Jan 24, 2016·Applied and Environmental Microbiology·Xin YanMary E Lidstrom
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Jul 1, 2016·World Journal of Microbiology & Biotechnology·Ludmila Chistoserdova
Oct 25, 2016·Nature Communications·Karthik AnantharamanJillian F Banfield
Dec 29, 2016·Proceedings of the National Academy of Sciences of the United States of America·Sascha M B KrauseMary E Lidstrom
Jun 21, 2017·Journal of Bacteriology·Zheng Yu, Ludmila Chistoserdova

❮ Previous
Next ❯

Citations

Jan 18, 2019·Molecular Microbiology·Andrea M OchsnerJulia A Vorholt
Apr 25, 2019·Chembiochem : a European Journal of Chemical Biology·Emily R FeatherstonJoseph A Cotruvo
Aug 3, 2019·FEMS Microbiology Ecology·Karen M HoughtonIan R McDonald
Oct 13, 2019·Applied and Environmental Microbiology·Carl-Eric WegnerKirsten Küsel
May 6, 2020·The Journal of Biological Chemistry·Nathan M GoodN Cecilia Martinez-Gomez
Dec 14, 2018·Frontiers in Microbiology·Ilya R AkberdinMarina G Kalyuzhnaya
Feb 16, 2020·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Bérénice JahnLena J Daumann
Oct 10, 2020·FEMS Microbiology Letters·Jennifer B GlassKaren H Johannesson
Sep 27, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Emily R Featherston, Joseph A Cotruvo
Jul 29, 2021·Environmental Microbiology·Andrew T Crombie
Jul 8, 2021·Microbiome·Obulisamy Parthiba KarthikeyanDeepak Kumaresan
Nov 6, 2021·Frontiers in Microbiology·Ola AlessaAkio Tani

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Protein Assay

Software Mentioned

BLAST
MEGA7
GraphPad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.