PMID: 11342009May 9, 2001Paper

Lanthanide ions induce hydrolysis of hemoglobin-bound 2,3-diphosphoglycerate (2,3-DPG), conformational changes of globin and bidirectional changes of 2,3-DPG-hemoglobin's oxygen affinity

Biochimica Et Biophysica Acta
Y ChengK Wang

Abstract

The changes in structure and function of 2,3-diphosphoglycerate-hemoglobin (2,3-DPG-Hb) induced by Ln(3+) binding were studied by spectroscopic methods. The binding of lanthanide cations to 2,3-DPG is prior to that to Hb. Ln(3+) binding causes the hydrolysis of either one from the two phosphomonoester bonds in 2,3-DPG non-specifically. The results using the ultrafiltration method indicate that Ln(3+) binding sites for Hb can be classified into three categories: i.e. positive cooperative sites (N(I)), non-cooperative strong sites (N(S)) and non-cooperative weak sites (N(W)) with binding constants in decreasing order: K(I)>K(S)>K(W). The total number of binding sites amounts to about 65 per Hb tetramer. Information on reaction kinetics was obtained from the change of intrinsic fluorescence in Hb monitored by stopped-flow fluorometry. Fluctuation of fluorescence dependent on Ln(3+) concentration and temperature was observed and can be attributed to the successive conformational changes induced by Ln(3+) binding. The results also reveal the bidirectional changes of the oxygen affinity of Hb in the dependence on Ln(3+) concentration. At the range of [Ln(3+)]/[Hb]<2, the marked increase of oxygen affinity (P(50) decrease) with the Ln...Continue Reading

References

Apr 5, 1979·Journal of Molecular Biology·J Baldwin, C Chothia
Mar 1, 1978·The Biochemical Journal·J G Gilman, G J Brewer
May 1, 1989·Archives of Biochemistry and Biophysics·P J Stankiewicz
Mar 1, 1971·The Journal of Clinical Investigation·A J BellinghamC Lenfant
Mar 28, 1980·Biochemical and Biophysical Research Communications·R E HirschR L Nagel
Aug 31, 1982·Biochemical and Biophysical Research Communications·H SakuraiS Shimomura
Jan 1, 1981·Methods in Enzymology·A Riggs
Jun 17, 1994·Journal of Molecular Biology·M F PerutzD Williamson
Oct 2, 1998·Journal of Inorganic Biochemistry·D XueK Wang
Nov 11, 1999·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Y ChengK Wang
Jul 7, 1956·Nature·R B HUNTER, W WALKER
Jul 1, 1942·The Journal of Clinical Investigation·S B BeaserL Vandam

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Citations

Sep 20, 2011·Journal of Photochemistry and Photobiology. B, Biology·Hongxia ChengGuolin Zou
Mar 30, 2010·Journal of Photochemistry and Photobiology. B, Biology·Paulami MandalTapan Ganguly
Apr 20, 2016·Journal of Biomolecular Structure & Dynamics·Paromita SealRajen Haldar
Nov 21, 2007·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Shaofen GuoXiaohua Huang
Nov 4, 2021·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Zhenxing ChiXunuo Zhang

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