PMID: 6972237Jun 1, 1981Paper

Lanthanide probes in biological systems: characterization of luminescence excitation spectra of terbium complexes with proteins

Biophysical Chemistry
J De JerseyR B Martin

Abstract

Upon irradiation in the ultraviolet region aromatic chromophores may transfer energy to a nearby Tb3+, which in turn emits a green phosphorescence. This paper reports the characterization of the ultraviolet excitation spectra of aromatic chromophores capable of transferring energy to Tb3+ by monitoring of the green Tb3+ emission in the 540-550 nm region. Results are included for complexes containing phenyl, hydroxyphenyl, indole, and catechol chromophores. Characteristic excitation spectra are presented for the aromatic chromophores occurring as side chains in proteins. Though it is preferable to compare entire excitation spectra, the ratio of intensities at 292 to 276 nm, R, is suggested as a useful diagnostic criterion. Numerical R valves are indicative of the following aromatic side chains as the energy donor to Tb3+: R less than 0.2, unionized tyrosine; R = 0.5 to 1.0, tryptophan; and R less than 1.8, ionized tyrosine. The phenylalanyl chromophore displays a definitive excitation spectrum at shorter wavelengths. For ovotransferrin R = 0.9 and comparison of the full excitation spectra suggests that it contains comparable contributions from both ionized tyrosine and tryptophan side chains. Some difficulties in obtaining relia...Continue Reading

References

May 31, 1977·Biochemistry·M EpsteinA Levitzki
May 28, 1975·Journal of the American Chemical Society·R K Boggess, R B Martin
Aug 1, 1978·Archives of Biochemistry and Biophysics·A D SherryG L Cottam
May 1, 1979·Quarterly Reviews of Biophysics·R B Martin, F S Richardson
Dec 8, 1976·Journal of the American Chemical Society·H G BrittainR B Martin
Jan 1, 1975·Bioinorganic Chemistry·R PradosR B Martin
Oct 15, 1975·FEBS Letters·T L MillerC M Kay
Feb 9, 1976·Biochemical and Biophysical Research Communications·H G BrittainC M Kay
Oct 22, 1974·Biochemistry·H Donato, R B Martin
Dec 1, 1980·Archives of Biochemistry and Biophysics·J De JerseyR B Martin

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Citations

Apr 6, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Changxia SunShufang Liu
Aug 15, 1986·Archives of Biochemistry and Biophysics·J Loscalzo, D Rabkin
Jul 1, 1991·Archives of Biochemistry and Biophysics·R L Tellam
Jun 23, 2012·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·N Ranjana DeviN Rajmuhon Singh
Aug 31, 1981·Biochemical and Biophysical Research Communications·P J MorleyS Boatman
Feb 28, 1983·Biochemical and Biophysical Research Communications·R G Canada

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