PMID: 2110832May 9, 1990Paper

Lanthanide(III)-phosphatidic acid complexes: binding site heterogeneity and phase separation

Biochimica Et Biophysica Acta
J Sun, M Petersheim

Abstract

The luminescent lanthanides are potentially useful probes of cation-induced events involving phospholipid membranes. In this work, the spectroscopic properties of Tb3+, Ce3+ and Eu3+ are shown to be complementary in defining three forms of complex with phosphatidic acid vesicles. Ce3+, in particular, is useful for studying dilute cation-lipid complexes because it has strong excitation bands in the near ultraviolet. In addition to providing a means for detecting chemically distinct forms of lanthanide-lipid complexes, the luminescence can be used to monitor cation-induced lateral segregation. Ce3+ to Tb3+ energy transfer was observed at lanthanide levels as low as 1:1000 Ln3+/phosphatidic acid, indicating clustering or phase separation. Initial clustering occurs on a subsecond timescale, followed by a much slower aggregation continuing for several minutes to hours. Addition of a chelator results in slow release of the lanthanides. In the case of the dioleoylphosphatidic acid complexes, release is bimodal and indicative of cation entrapment; dimyristoylphosphatidic acid complexes exhibit this behavior only at high temperatures. These observations are consistent with the relative tendencies of these two lipids to form the HII phas...Continue Reading

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Citations

May 15, 2002·European Journal of Pharmacology·Francisco J Rangel-GonzálezRicardo Miledi
Jun 6, 2000·Advanced Drug Delivery Reviews·G Cevc, H Richardsen
Jan 5, 2000·Biophysical Journal·J StruppeR R Vold
Apr 17, 2001·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·J R LakowiczI Gryczynski

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