Laparoscopic Fluorescent Visualization of the Ureter With Intravenous IRDye800CW

Journal of Minimally Invasive Gynecology
Melissa L KorbEben L Rosenthal

Abstract

Ureter injury is a serious complication of laparoscopic surgery. Current strategies to identify the ureters, such as placement of a ureteral stent, carry additional risks for patients. We hypothesize that the systemically injected near-infrared (NIR) dye IRDye800CW-CA can be used to visualize ureters intraoperatively. Adult female mixed-breed pigs weighing 24 to 41 kg (n = 2 per dose) were given a 30, 60, or 120 μg/kg systemic injection of IRDye800CW-CA. Using the Food and Drug Administration-cleared Pinpoint laparoscopic NIR system, images of the ureter and bladder were captured every 10 minutes for 60 minutes after injection. To determine the biodistribution of the dye, tissues were collected for ex vivo analysis with the Pearl Impulse system. ImageJ software was used to quantify fluorescence signal and signal-to-background ratio (SBR) for the intraoperative images. The ureter was identified in all pigs at each dose, with peak intensity reached by 30 minutes and remaining elevated throughout the duration of imaging (60 minutes). The 60 μg/kg dose was determined to be optimal for differentiating ureters according to absolute fluorescence (>60 counts/pixel) and SBR (3.1). Urine fluorescence was inversely related to plasma fluor...Continue Reading

References

Apr 1, 1994·Diseases of the Colon and Rectum·W N BothwellT L Dent
May 1, 1996·The Journal of the American Association of Gynecologic Laparoscopists·E C WoodM A Pelosi
Jan 16, 2002·JAMA : the Journal of the American Medical Association·Jane C WeeksUNKNOWN Clinical Outcomes of Surgical Therapy (COST) Study Group
Aug 5, 2004·BJU International·Steven BrandesJack McAninch
May 5, 2007·Journal of Biomedical Optics·Kristen E AdamsEva M Sevick-Muraca
Sep 18, 2007·The Journal of Urology·Eiichi TanakaJohn V Frangioni
Apr 9, 2010·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Milton V MarshallD Michael Olive
Mar 2, 2012·Korean journal of urology·Jeong Hyun ParkMoon Ki Jo
Mar 21, 2012·Archives of Surgery·Nandini C PalaniappaCelia M Divino
May 1, 2012·The Journal of Obstetrics and Gynaecology Research·Eiji KobayashiMasaaki Andou
Jul 11, 2012·Asian Journal of Endoscopic Surgery·G da SilvaS D Wexner
Feb 21, 2013·JAMA : the Journal of the American Medical Association·Jason D WrightDawn L Hershman
May 16, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Stijn KeereweerClemens W G M Löwik
May 30, 2013·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Kristine E DayEben L Rosenthal
Dec 24, 2013·The Journal of Surgical Research·Melissa L KorbEben L Rosenthal
Jan 1, 2014·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·M Z WilsonD B Stewart
Feb 11, 2014·Diseases of the Colon and Rectum·Rutger M ScholsLaurents P S Stassen
Dec 10, 2014·JSLS : Journal of the Society of Laparoendoscopic Surgeons·Jonathan P ShepherdTed Lee

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Citations

Nov 28, 2015·Techniques in Coloproctology·A G Franchini MelaniJ Marescaux
Aug 5, 2018·Journal of Surgical Oncology·Pietro MascagniMichele Diana
Jun 9, 2019·International Journal of Colorectal Disease·Stefanie M CroghanDes C Winter
Jun 20, 2019·Journal of Biomedical Optics·Richard W FarnamJonathan M Sorger
Oct 4, 2019·Lasers in Surgery and Medicine·Lung Wai LauJaepyeong Cha
Jul 28, 2020·Journal of Laparoendoscopic & Advanced Surgical Techniques. Part a·Mahdi Al-TaherMichele Diana
Jun 22, 2016·Current Opinion in Obstetrics & Gynecology·Austin D Findley, M Jonathon Solnik
May 30, 2019·Frontiers in Pharmacology·Pieterjan Debie, Sophie Hernot
Oct 28, 2018·European Journal of Medicinal Chemistry·Dan WuDonal F O'Shea
Jul 3, 2020·Journal of Minimally Invasive Gynecology·Warner K HuhKenneth H Kim
Mar 8, 2018·Bioorganic & Medicinal Chemistry Letters·Jaepyeong ChaMartin J Schnermann
May 13, 2021·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Hiroshi FushikiAkira Suwa
Jul 7, 2018·Molecular Pharmaceutics·Sakkarapalayam M MahalingamPhilip S Low
May 24, 2018··Alexander L. VahrmeijerLabrinus van Manen

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