Laquinimod dampens IL-1β signaling and Th17-polarizing capacity of monocytes in patients with MS.

Neurology. Neuroimmunology and Neuroinflammation
Sinah EngelFelix Luessi

Abstract

To assess the impact of laquinimod treatment on monocytes and to investigate the underlying immunomodulatory mechanisms in MS. In this cross-sectional study, we performed in vivo and in vitro analyses of cluster of differentiation (CD14+) monocytes isolated from healthy donors (n = 15), untreated (n = 13), and laquinimod-treated patients with MS (n = 14). Their frequency and the expression of surface activation markers were assessed by flow cytometry and the viability by calcein staining. Cytokine concentrations in the supernatants of lipopolysaccharide (LPS)-stimulated monocytes were determined by flow cytometry. The messenger ribonucleic acid (mRNA) expression level of genes involved in cytokine expression was measured by quantitative PCR. The LPS-mediated nuclear factor kappa-light-chain-enhancer of activated B-cell (NF-κB) activation was determined by the quantification of the phosphorylation level of the p65 subunit. Laquinimod-treated monocytes were cocultured with CD4+ T cells, and the resulting cytokine production was analyzed by flow cytometry after intracellular cytokine staining. The interleukin (IL)-17A concentration of the supernatant was assessed by ELISA. Laquinimod did not alter the frequency or viability of cir...Continue Reading

References

Jul 2, 1998·Clinical and Experimental Immunology·G Fingerle-RowsonH W Ziegler-Heitbrock
Mar 29, 2001·Trends in Immunology·C A Chambers
Mar 23, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·David H MunnAndrew L Mellor
Jan 10, 2009·Annual Review of Immunology·Thomas KornVijay K Kuchroo
Mar 16, 2012·The New England Journal of Medicine·Giancarlo ComiUNKNOWN ALLEGRO Study Group
Jul 4, 2012·The American Journal of Pathology·Manoj K MishraV Wee Yong
Jul 7, 2012·Acta Neuropathologica·Wolfgang BrückChristiane Wegner
Mar 23, 2013·Brain : a Journal of Neurology·Valérie JolivelAri Waisman
Aug 8, 2013·Journal of Neuroimmunology·Brett T LundLeslie P Weiner
Sep 14, 2013·Journal of Neurology, Neurosurgery, and Psychiatry·Massimo FilippiUNKNOWN ALLEGRO Study Group
Nov 1, 2013·Molecular Immunology·Donghee Kim, Jae Young Kim
Mar 19, 2014·Immunology and Cell Biology·Delgertsetseg ChuluundorjAnne Camille La Flamme
Apr 1, 2014·Experimental Neurology·Christopher SieMeike Mitsdoerffer
Oct 5, 2014·Gut·Geert D'HaensUNKNOWN Laquinimod for Crohn's Disease Investigators
Jan 30, 2016·Journal of Neurochemistry·Lucianne DobsonSarah J Tabrizi
Apr 5, 2016·Drug Design, Development and Therapy·Jan Thöne, Ralf A Linker
May 28, 2016·Neurology. Neuroimmunology and Neuroinflammation·Nadine KramannWolfgang Brück
Jun 15, 2016·Experimental Neurology·Dagmar E EhrnhoeferMichael R Hayden
Aug 16, 2016·Nature Reviews. Neurology·Manoj K Mishra, V Wee Yong
Nov 3, 2016·Clinical and Experimental Immunology·A P JonesP H Hart
Mar 1, 2017·International Journal of MS Care·Per Soelberg SørensenVolker Knappertz
Jun 7, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chih-Chung Lin, Brian T Edelson
Jan 7, 2018·Journal of Neuroinflammation·Antonietta GentileGeorgia Mandolesi
Mar 8, 2018·Multiple Sclerosis : Clinical and Laboratory Research·Kerstin GöbelSven G Meuth
Mar 25, 2018·Scientific Reports·Céline CaravagnaFranck Debarbieux
Mar 27, 2018·Lancet·Alan J ThompsonOlga Ciccarelli
Aug 22, 2018·Nature Neuroscience·Giuseppe LocatelliMartin Kerschensteiner

❮ Previous
Next ❯

Citations

Oct 29, 2021·Glia·Lindsey H Forbes, Veronique E Miron

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
PCR
flow
ELISA

Software Mentioned

ALLEGRO
ARPEGGIO
FlowJo

Related Concepts

Related Feeds

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.