Large and small proteoglycans of osteoarthritic and rheumatoid articular cartilage

Arthritis and Rheumatism
Gabriella Cs-SzaboTibor T Glant

Abstract

To identify characteristic changes in large aggregating (aggrecan) and small proteoglycan (PG) populations in articular cartilages during osteoarthritis (OA) and rheumatoid arthritis (RA). Aggrecan populations in guanidine extracts of femoral condylar cartilages of 46 OA and 8 RA patients who underwent total knee arthroplasty, as well as of 2 fetuses and 6 normal adults, were separated in agarose-polyacrylamide composite gels. Small PGs (biglycan, decorin, and fibromodulin) in the same extracts were analyzed in 12% polyacrylamide gels. Gels were stained or electrophoretically transferred and probed with antibodies to aggrecan epitopes and to small PGs. Epitope contents of the samples were also compared by inhibition radioimmunoassay. There were significant differences found among normal and diseased samples in their electrophoretic mobilities, band distributions, and antibody staining. OA and especially RA samples were heavily degraded, lacked certain aggrecan populations, and contained fewer keratan sulfate and chondroitin-6-sulfate epitopes compared with normal samples. Levels of chondroitin-4-sulfate and "fetal-type" epitopes were elevated in the OA samples compared with the normal ones. More core proteins of small PGs were ...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1978·The Biochemical Journal·S InerotS E Olsson
Jul 1, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·J P PelletierC J Malemud
Sep 1, 1992·Arthritis and Rheumatism·P Witsch-PrehmH Kresse
May 1, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A RatcliffeT E Hardingham
Sep 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C A PooleJ R Schofield
Jun 1, 1991·Seminars in Arthritis and Rheumatism·D D Dean
Sep 1, 1991·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·S InerotL Audell
Oct 1, 1991·Current Opinion in Cell Biology·R Flaumenhaft, D B Rifkin
May 1, 1987·Arthritis and Rheumatism·V C Hascall, T T Glant
Nov 1, 1989·Arthritis and Rheumatism·L S LohmanderD Heinegård
Jul 15, 1989·The Biochemical Journal·L I Melching, P J Roughley
Apr 15, 1983·Analytical Biochemistry·E W Willoughby, A Lambert
Feb 1, 1983·The Biochemical Journal·M T BaylissS Y Ali
Oct 15, 1993·The Biochemical Journal·P J RoughleyJ S Mort
Oct 1, 1962·Analytical Biochemistry·T BITTER, H M MUIR

❮ Previous
Next ❯

Citations

Jan 1, 1996·Pathology Oncology Research : POR·Edit I BuzásTibor T Glant
Jan 2, 2004·Aging Clinical and Experimental Research·Florenzo Iannone, Giovanni Lapadula
Feb 24, 2012·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Sharon BrownSally Roberts
Mar 31, 2005·Biochemical and Biophysical Research Communications·Jochen G HofstaetterMelvin J Glimcher
Apr 25, 2000·Archives of Oral Biology·M Del SantoR J Hinton
May 13, 1999·Progress in Histochemistry and Cytochemistry·K Ostergaard, D M Salter
Mar 12, 2003·Biochimie·Nickoletta PapageorgakopoulouConstantine P Tsiganos
Mar 29, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Robert SztrolovicsPeter J Roughley
Dec 11, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Alexander GiureaRobert L Sah
Sep 1, 1996·Matrix Biology : Journal of the International Society for Matrix Biology·A HuttenlocherC H Damsky
Dec 2, 2010·Nature Reviews. Rheumatology·Dick Heinegård, Tore Saxne
May 29, 2004·Tissue Engineering·Kenichi KajitaniHiroko Kataoka
Jul 19, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Jun QinLiao-Bin Chen
Jun 14, 2000·American Journal of Respiratory and Critical Care Medicine·N VenkatesanM S Ludwig
Dec 23, 1998·Connective Tissue Research·N A VisserG P van Kampen
Dec 17, 2009·Connective Tissue Research·Jack L LewisJennifer J Westendorf
Dec 21, 2006·Autoimmunity·Edit I BuzásHans-Joachim Gabius
Jan 18, 2016·Archives of Oral Biology·Jitjiroj IttichaicharoenSiriporn C Chattipakorn
Jan 1, 1997·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·S H LeeT T Glant
Mar 6, 2012·Bone·Carla R Scanzello, Steven R Goldring
May 6, 2008·Best Practice & Research. Clinical Rheumatology·Johanne Martel-PelletierPeter J Roughley
Mar 1, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hirotaka HaroDan M Spengler
May 26, 2012·The Journal of Comparative Neurology·Jae-Hyuk YiHerbert M Geller
Nov 10, 2004·Matrix Biology : Journal of the International Society for Matrix Biology·Pilar LorenzoDick Heinegård
Feb 21, 2006·Biochimie·D H VyniosA P Andonopoulos
Jun 5, 1999·Rheumatic Diseases Clinics of North America·F A Wollheim
Aug 9, 2005·Seminars in Arthritis and Rheumatism·Piercarlo Sarzi-PuttiniBianca Canesi
Jun 12, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Wenhua LiuGabriella Cs-Szabo
Feb 25, 1998·Journal of the American Geriatrics Society·S M Ling, J M Bathon

❮ Previous
Next ❯

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