Large but not small intrahepatic bile ducts are involved in secretin-regulated ductal bile secretion

The American Journal of Physiology
Gianfranco AlpiniGene Lesage

Abstract

We have shown that agonist-regulated ductal secretion is limited to large cholangiocytes. To directly study cholangiocyte heterogeneity along the length of the normal biliary tree, we defined the genetic and functional expression of agonist-induced ductal secretion in intrahepatic bile duct units (IBDU) of different sizes. Small IBDU (< 15-microns diam) were separated from large IBDU (> or = 15-microns diam), and then ducts of different sizes were characterized by morphometric analysis, gene expression, secretin-induced adenosine 3',5'-cyclic monophosphate (cAMP) synthesis, and secretion by change in luminal size in response to agonists. IBDU diameters ranged from 11 to 65 microns. Secretin increased ductal secretion solely in large IBDU. Forskolin induced a modest increase in ductal secretion in small IBDU but markedly increased ductal secretion in large IBDU. Secretion increased Cl-/HCO3- exchanger activity and cAMP levels in large but not small IBDU. Secretin receptor and Cl-/HCO3 exchanger mRNAs were detected only in large IBDU. We propose that agonist-induced ductal secretion occurs in large (> or = 15-microns diam) but not small (< 15-microns diam) intrahepatic ducts.

Citations

Jan 24, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Gianfranco AlpiniGene LeSage
Jun 15, 2006·World Journal of Gastroenterology : WJG·Xuefeng XiaGene LeSage
May 16, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mario StrazzaboscoGianfranco Alpini
Apr 25, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Romina MancinelliGianfranco Alpini
Jul 16, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Anastasia RenziGianfranco Alpini
Feb 8, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Romina MancinelliGianfranco Alpini
Sep 13, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yuyan HanGianfranco Alpini
Feb 20, 2020·Cells·Keisaku SatoGianfranco Alpini
Aug 24, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·N KannoG Alpini
Oct 22, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Luca MarucciGene LeSage
Apr 5, 2003·American Journal of Physiology. Cell Physiology·Ai-Yu GongNicholas F LaRusso
Feb 11, 2009·Laboratory Investigation; a Journal of Technical Methods and Pathology·Shannon S GlaserGianfranco D Alpini
Jun 15, 2006·World Journal of Gastroenterology : WJG·Shannon GlaserGianfranco Alpini
Oct 13, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Giammarco FavaGianfranco Alpini
Apr 27, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Nan WuGianfranco Alpini
Jul 12, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·S K SinghJ L Boyer
Mar 20, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·J M McGillA P Stansfield
Dec 31, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shannon GlaserGianfranco Alpini
May 30, 2008·American Journal of Physiology. Cell Physiology·Heather FrancisGianfranco Alpini
Jul 17, 2013·Experimental Biology and Medicine·Yuyan HanAntonio Franchitto
May 21, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shannon GlaserGianfranco Alpini
Mar 17, 2010·Journal of Cell Death·Fuquan YangShannon S Glaser
Jul 7, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Tianhao ZhouGianfranco Alpini
Jun 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·G LesageG Alpini
Jul 20, 2012·American Journal of Physiology. Renal Physiology·Xiaofang WangVicente E Torres
Oct 10, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Romina MancinelliEugenio Gaudio
Oct 3, 2018·International Journal of Molecular Sciences·Debora Maria GiordanoMarco Marzioni
Jan 29, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Gianfranco AlpiniShannon Glaser
Jun 26, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shannon GlaserGianfranco Alpini
Jun 30, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lindsey KennedyGianfranco Alpini
Jun 15, 2006·World Journal of Gastroenterology : WJG·Jesús-M BanalesJuan-F Medina
Aug 2, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Nan WuGianfranco Alpini
Mar 4, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Floris J M RoosLuc J W van der Laan
Feb 2, 2011·The American Journal of Pathology·Md Kamruzzaman MunshiShannon S Glaser
Feb 16, 2010·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Shannon S GlaserEugenio Gaudio

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