Large Changes in Protonation of Weak Polyelectrolyte Brushes with Salt Concentration-Implications for Protein Immobilization

The Journal of Physical Chemistry Letters
Gustav Ferrand-Drake Del CastilloAndreas Dahlin

Abstract

We report for the first time that the protonation behavior of weak polyelectrolyte brushes depends very strongly on ionic strength. The pKa changes by one pH step per order of magnitude in salt concentration. For low salt concentrations (∼1 mM), a very high pH is required to deprotonate a polyacidic brush and a very low pH is required to protonate a polybasic brush. This has major consequences for interactions with other macromolecules, as the brushes are actually almost fully neutral when believed to be charged. We propose that many previous studies on electrostatic interactions between polyelectrolytes and proteins have, in fact, looked at other types of intermolecular forces, in particular, hydrophobic interactions and hydrogen bonds.

References

Apr 24, 2002·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·Y TranM Stamm
Mar 22, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Oliver Hollmann, Claus Czeslik
Apr 19, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Jinhua DaiMerlin L Bruening
Jan 24, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Oliver HollmannClaus Czeslik
Mar 16, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Andy KusumoRobert D Tilton
Feb 2, 2008·Science·Seunghwan Lee, Nicholas D Spencer
Dec 31, 2008·Journal of the American Chemical Society·Marina PrivmanEvgeny Katz
Feb 27, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Rong DongChristopher K Ober
Dec 17, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Tsz Kin TamEvgeny Katz
Jan 23, 2010·Nature Materials·Martien A Cohen StuartSergiy Minko
Oct 12, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·M Gabriella SantonicolaG Julius Vancso
Mar 24, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Nicolas Schüwer, Harm-Anton Klok
Jan 31, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Joshua D WillottErica J Wanless
Sep 23, 2014·Acta Biomaterialia·M F DelcroixC C Dupont-Gillain
Aug 12, 2015·The Journal of Physical Chemistry Letters·Evgeny KatzArtem Melman
May 6, 2016·Nature Nanotechnology·Andreas KüchlerPeter Walde
Dec 17, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Cemil YigitJoachim Dzubiella
Jun 20, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Jian ZhangGuangming Liu
May 29, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Guangming Liu
Feb 12, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Gustav Ferrand-Drake Del CastilloAndreas Dahlin
Aug 16, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Xin XuJing Yu
Aug 14, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hanna GustafssonPeter Walde
May 7, 2020·Chemical Communications : Chem Comm·G Ferrand-Drake Del CastilloAndreas Dahlin

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Citations

Feb 25, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Mikhail Y LaktionovOleg V Borisov
Apr 15, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·John AnderssonAndreas Dahlin
Jul 9, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Brian K Wilson, Robert K Prud'homme

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Methods Mentioned

BETA
surface plasmon resonance
quartz crystal microbalance
neutron reflectometry

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