Large-field-of-view optical resolution photoacoustic microscopy

Optics Express
Wei QinLei Xi

Abstract

The use of existing optical resolution photoacoustic microscopy (ORPAM) has been limited to small organs or part of large organs due to the millimeter-scale field of view (FOV) in both lateral and axial directions. Here, we report a large-field-of-view ORPAM (L-ORPAM) using a combination of a new scanning mechanism and an ultrafast pulsed laser. Phantom and in vivo experiments show that L-ORPAM has a spatial FOV of 40 mm in lateral and 12 mm in axial, which expends the effective imaging domain to one order that of existing ORPAMs. To show the advantages of L-ORPAM, we apply it to imaging vasculatures of both brain and ears simultaneously in mice, and to visualizing intestinal vasculatures in rats. The result suggests that L-ORPAM has sufficient contrast, resolution and spatial FOV to carry out studies of large rodents.

References

Aug 3, 2010·Nature Methods·Vasilis Ntziachristos
Aug 7, 2012·Interface Focus·Paul Beard
Apr 2, 2014·Optics Letters·Lei LiLihong V Wang
Jan 6, 2015·Optica·Liren ZhuLihong V Wang
Jan 13, 2015·Biomedical Optics Express·Wei SongLiang Song
Jul 29, 2016·Nature Methods·Lihong V Wang, Junjie Yao
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Jan 28, 2017·Journal of Biophotonics·Weizhi QiLei Xi
Nov 16, 2017·Optics Letters·Heng GuoLei Xi

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Citations

Oct 28, 2019·Sensors·Xiangwei LinLidai Wang
Apr 3, 2020·Visual Computing for Industry, Biomedicine and Art·Qiwen XuHuabei Jiang
Oct 16, 2018·Biomedical Optics Express·Bangxin LanJunjie Yao
May 27, 2021·Visual Computing for Industry, Biomedicine and Art·Yongchao Wang, Lei Xi
Jul 17, 2021·Clinical, Cosmetic and Investigational Dermatology·Dong ZhangAixi Yu
Aug 26, 2021·Photoacoustics·Jiangbo ChenLidai Wang

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