Large functional range of steady-state levels of nuclear and mitochondrial transcripts coding for the subunits of the human mitochondrial OXPHOS system

Genome Research
H DuborjalJ Issartel


We have measured, by reverse transcription and real-time quantitative PCR, the steady-state levels of the mitochondrial and nuclear transcripts encoding several subunits of the human oxidative phosphorylation (OXPHOS) system, in different normal tissues (muscle, liver, trachea, and kidney) and in cultured cells (normal fibroblasts, 143B osteosarcoma cells, 143B206 rho(0) cells). Five mitochondrial transcripts and nine nuclear transcripts were assessed. The measured amounts of these OXPHOS transcripts in muscle samples corroborated data obtained by others using the serial analysis of gene expression (SAGE) method to appraise gene expression in the same type of tissue. Steady-state levels for all the transcripts were found to range over more than two orders of magnitude. Most of the time, the mitochondrial H-strand transcripts were present at higher levels than the nuclear transcripts. The mitochondrial L-strand transcript ND6 was usually present at a low level. Cultured 143B cells contained significantly reduced amounts of mitochondrial transcripts in comparison with the tissue samples. In 143B206 rho(0) cells, fully depleted of mitochondrial DNA, the levels of nuclear OXPHOS transcripts were not modified in comparison with the ...Continue Reading


Jan 1, 1975·Advances in Enzyme Regulation·D Pette, G Dölken
Mar 9, 1987·FEBS Letters·P CantatoreA de Montalvo
Jan 13, 1984·Biochemical and Biophysical Research Communications·P CantatoreC Saccone
Jul 1, 1994·Clinica Chimica Acta; International Journal of Clinical Chemistry·P RustinA Munnich
Apr 15, 1994·Biochemical and Biophysical Research Communications·H L GarstkaR J Wiesner
Aug 1, 1996·Annals of Neurology·P KaufmannE A Schon
Sep 1, 1996·Archives of Biochemistry and Biophysics·M K ConnorD A Hood
Apr 1, 1997·Journal of Bioenergetics and Biomembranes·R W TaylorD M Turnbull
Aug 26, 1998·Biochimica Et Biophysica Acta·S C LearyC D Moyes
Jan 8, 1999·Biochemical and Biophysical Research Communications·J L LoeffenJ A Smeitink
Mar 17, 1999·Biochimica Et Biophysica Acta·J W Taanman
Aug 7, 1999·The Journal of Biological Chemistry·A HeddiD C Wallace
Apr 21, 2001·The Journal of Biological Chemistry·E LinG A Freyer


Dec 13, 2005·Journal of Bioenergetics and Biomembranes·Arnaud ChevrollierGeorges Stepien
Oct 6, 2011·The Journal of Biological Chemistry·Lijun LiuMarcus P Cooper
Feb 1, 2005·Trends in Genetics : TIG·Marco SardielloGraziano Pesole
May 31, 2012·The Biochemical Journal·Joanna Rorbach, Michal Minczuk
Apr 25, 2009·Biochimica Et Biophysica Acta·Fimmie ReineckeF H van der Westhuizen
Nov 3, 2011·Biochimica Et Biophysica Acta·Dan Bar-YaacovDan Mishmar
Jan 24, 2009·Methods in Enzymology·Asuteka NagaoTsutomu Suzuki
Sep 20, 2018·Annual Review of Genetics·R Stefan IsaacL Stirling Churchman
Jan 14, 2020·Annual Review of Biophysics·Linhao RuanRong Li
Apr 17, 2014·The Journal of Experimental Biology·Katharina Bremer, C D Moyes

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