Large quantity production with extreme convenience of human SDF-1alpha and SDF-1beta by a Sendai virus vector

FEBS Letters
C MoriyaY Nagai

Abstract

We describe a robust expression of human stromal cell-derived factor-1alpha (SDF-1alpha) and SDF-1beta, the members of CXC-chemokine family, with a novel vector system based upon Sendai virus, a non-segmented negative strand RNA virus. Recombinant SDF-1alpha and SDF-1beta were detected as a major protein species in culture supernatants, reached as high as 10 microg/ ml. This remarkable enrichment of the products allowed us to use even the crude supernatants as the source for biological and antiviral assays without further concentration nor purification and will thus greatly facilitate to screen their genetically engineered derivatives.

References

Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J P Tam
Jun 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·H Hanafusa
Mar 1, 1994·AIDS Research and Human Retroviruses·S OkaK Shimada
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·T NagasawaT Kishimoto
Oct 3, 1996·Nature·F Arenzana-SeisdedosM Baggiolini
Sep 1, 1996·The Journal of Experimental Medicine·C C BleulT A Springer
Jun 1, 1996·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·A KatoY Nagai
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·A L EdingerR W Doms
May 19, 1997·The Journal of Experimental Medicine·K TachibanaT Nagasawa
Jul 1, 1997·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·D YuY Nagai
Mar 20, 1998·Archives of Virology·X XinY Nagai

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Citations

Aug 24, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Y OhnishiY Mitsui
Oct 21, 1999·Microbiology and Immunology·Y Nagai, A Kato
Jan 12, 2008·Journal of the American Society for Mass Spectrometry·Roxana E IacobKenneth B Tomer
Jun 26, 2003·The Journal of Gene Medicine·Michael BitzerWolfgang J Neubert
Aug 31, 1999·The Journal of General Virology·M D BaronT Barrett

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