Large scale biobanking of blood - the importance of high density sample processing procedures

Journal of Proteomics
Johan MalmGyörgy Marko-Varga

Abstract

The aim of this study is a novel automated sample-processing concept for future proteomics and clinical research, performing patient studies from resulting blood fractions in various disease areas. Another aim is biobank storage of small sample volumes, where each sample aliquot can be used for a dedicated clinical analysis and end-point measurement in order to preserve sample integrity and value over time. 96 and 384 format sample storage tube systems were utilized for preservation and archiving of clinical patient samples. Automated sample processing and aliquoting were achieved using robotic liquid handling instrumentation, followed by biomarker assay quantitations. Sample workflow was documented and tracked by Nautilus LIMS. Validation by repetitive processing and analysis confirmed the reliability of automated high density 384 format aliquoting. This high density scaling allows for reproducible aliquoting of 70-μL volumes of blood. Plasma with EDTA, Li-heparin, and citrate, as anti-coagulants, fractioned along with the buffy coat (leukocytes) and the erythrocyte fraction. Large scale processing of 11,000 sample aliquots resulted in a 99.8% process fulfillment. Our results demonstrate that robust results can be generated fr...Continue Reading

References

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Citations

Feb 8, 2013·Clinical and Translational Medicine·György Marko-Varga
Mar 1, 2013·Clinical and Translational Medicine·Charlotte WelinderGyörgy Marko-Varga
Oct 20, 2012·Journal of Proteomics·Lello Zolla, Angelo D'Alessandro
Feb 18, 2016·Biopreservation and Biobanking·Rolf MullerUNKNOWN International Society for Biological and Environmental Repositories (ISBER) Biospecimen Science Working Group
Jan 31, 2015·Clinical and Translational Medicine·Toshihide NishimuraGyörgy Marko-Varga
Jul 29, 2015·Journal of Proteomics·Åsa M WheelockUNKNOWN EuPA Biobank Initiative Group
May 17, 2015·Clinical Chemistry·Christina Ellervik, Jim Vaught
Mar 9, 2017·Expert Review of Proteomics·Kiyonaga FujiiToshihide Nishimura
Jul 30, 2021·Clinical and Translational Medicine·Lazaro Hiram BetancourtGyörgy Marko-Varga
May 25, 2013·Journal of Proteome Research·Johan MalmGyörgy Marko-Varga

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