Large-scale clinical validation of biomarkers for pancreatic cancer using a mass spectrometry-based proteomics approach

Oncotarget
Jisook ParkSoo-Youn Lee

Abstract

We performed an integrated analysis of proteomic and transcriptomic datasets to develop potential diagnostic markers for early pancreatic cancer. In the discovery phase, a multiple reaction monitoring assay of 90 proteins identified by either gene expression analysis or global serum proteome profiling was established and applied to 182 clinical specimens. Nine proteins (P < 0.05) were selected for the independent validation phase and quantified using stable isotope dilution-multiple reaction monitoring-mass spectrometry in 456 specimens. Of these proteins, four proteins (apolipoprotein A-IV, apolipoprotein CIII, insulin-like growth factor binding protein 2 and tissue inhibitor of metalloproteinase 1) were significantly altered in pancreatic cancer in both the discovery and validation phase (P < 0.01). Moreover, a panel including carbohydrate antigen 19-9, apolipoprotein A-IV and tissue inhibitor of metalloproteinase 1 showed better performance for distinguishing early pancreatic cancer from pancreatitis (Area under the curve = 0.934, 86% sensitivity at fixed 90% specificity) than carbohydrate antigen 19-9 alone (71% sensitivity).Overall, we present the panel of robust biomarkers for early pancreatic cancer diagnosis through bio...Continue Reading

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Citations

Apr 18, 2018·Oncotarget·Cristina Jimenez-LunaOctavio Caba
Jul 28, 2019·Cancers·Iranzu González-BorjaJoaquin Fernández-Irigoyen
Aug 7, 2020·Expert Review of Proteomics·Paula MeleadyMartin Clynes
Sep 20, 2017·Expert Review of Proteomics·Hyunsoo KimYoungsoo Kim
Dec 13, 2018·Expert Opinion on Investigational Drugs·Sreenivasa ChandanaDaruka Mahadevan
Dec 12, 2020·Computational and Structural Biotechnology Journal·Wu-Hu ZhangLiang Liu
Sep 9, 2019·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Felipe Paiva FonsecaPablo Agustin Vargas
Nov 30, 2019·Cancer Letters·Damiano Caputo, Giulio Caracciolo
Apr 4, 2021·International Journal of Molecular Sciences·Randa G Hanna-SawiresRob A E M Tollenaar
Jan 22, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Tomasz KowalczykCoral Barbas
Mar 20, 2021·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sidsel C LindgaardJulia S Johansen

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Methods Mentioned

BETA
surgical resection
proteomic
electrophoresis
ELISA
protein assays

Software Mentioned

MedCalc
SEQUEST

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