Large-scale comparative analysis of cytogenetic markers across Lepidoptera.

Scientific Reports
Irena ProvazníkováPetr Nguyen

Abstract

Fluorescence in situ hybridization (FISH) allows identification of particular chromosomes and their rearrangements. Using FISH with signal enhancement via antibody amplification and enzymatically catalysed reporter deposition, we evaluated applicability of universal cytogenetic markers, namely 18S and 5S rDNA genes, U1 and U2 snRNA genes, and histone H3 genes, in the study of the karyotype evolution in moths and butterflies. Major rDNA underwent rather erratic evolution, which does not always reflect chromosomal changes. In contrast, the hybridization pattern of histone H3 genes was well conserved, reflecting the stable organisation of lepidopteran genomes. Unlike 5S rDNA and U1 and U2 snRNA genes which we failed to detect, except for 5S rDNA in a few representatives of early diverging lepidopteran lineages. To explain the negative FISH results, we used quantitative PCR and Southern hybridization to estimate the copy number and organization of the studied genes in selected species. The results suggested that their detection was hampered by long spacers between the genes and/or their scattered distribution. Our results question homology of 5S rDNA and U1 and U2 snRNA loci in comparative studies. We recommend the use of histone H...Continue Reading

References

Jun 1, 1975·Canadian Journal of Genetics and Cytology. Journal Canadien De Génétique Et De Cytologie·G E Steiniger, A B Mukherjee
Sep 1, 1976·Nucleic Acids Research·N Blin, D W Stafford
Jan 1, 1983·Annual Review of Genetics·R MaxsonT Mohun
Feb 5, 1999·Chromosoma·J E SchienmanL D Strausbaugh
Apr 3, 2003·Genome Génome / Conseil National De Recherches Canada·Cheryl D ProkopowichTeresa J Crease
Aug 9, 2003·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Rita BarzottiAngela Rocchi
Apr 2, 2005·Genetics·Atsuo YoshidoKen Sahara
Aug 17, 2005·Cytogenetic and Genome Research·U Claussen
Jan 5, 2006·Genome Génome / Conseil National De Recherches Canada·Iva FukováFrantisek Marec
Nov 11, 2006·RNA·Stephen M MountWojciech Makalowski
Nov 26, 2008·Journal of Molecular Evolution·Manuela MarzPeter F Stadler
May 7, 2010·Proceedings. Biological Sciences·Marko MutanenLauri Kaila
Jun 17, 2010·BMC Genetics·Andréia B PolettoCesar Martins
Jan 12, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·D C Cabral-de-MelloC Martins
Apr 25, 2012·Chromosoma·Sònia GarciaAleš Kovařík
Apr 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Petr NguyenFrantišek Marec
Aug 14, 2013·BMC Evolutionary Biology·Octavio M Palacios-GimenezDiogo C Cabral-de-Mello
Jan 18, 2014·Evolution; International Journal of Organic Evolution·Michail RovatsosLukáš Kratochvíl
Dec 5, 2014·BMC Genetics·Leonela Z Carabajal PaladinoFrantišek Marec
May 20, 2015·BMC Evolutionary Biology·Jindra ŠíchováFrantišek Marec

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