Large Scale Discovery and De Novo-Assisted Sequencing of Cationic Antimicrobial Peptides (CAMPs) by Microparticle Capture and Electron-Transfer Dissociation (ETD) Mass Spectrometry

Journal of Proteome Research
Melanie L JubaBarney M Bishop

Abstract

The identification and sequencing of novel cationic antimicrobial peptides (CAMPs) have proven challenging due to the limitations associated with traditional proteomics methods and difficulties sequencing peptides present in complex biomolecular mixtures. We present here a process for large-scale identification and de novo-assisted sequencing of newly discovered CAMPs using microparticle capture followed by tandem mass spectrometry equipped with electron-transfer dissociation (ETD). This process was initially evaluated and verified using known CAMPs with varying physicochemical properties. The effective parameters were then applied in the analysis of a complex mixture of peptides harvested from American alligator plasma using custom-made (Bioprospector) functionalized hydrogel particles. Here, we report the successful sequencing process for CAMPs that has led to the identification of 340 unique peptides and the discovery of five novel CAMPs from American alligator plasma.

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Citations

Sep 22, 2016·Expert Review of Proteomics·Osmel Fleitas MartinezOctávio Luiz Franco
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Aug 24, 2019·Protein Science : a Publication of the Protein Society·Faiza Hanif Waghu, Susan Idicula-Thomas
Dec 22, 2020·Mass Spectrometry Reviews·Albert T LebedevTatiana Y Samgina

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