Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae

Genome Biology
Jeanne BoyerBernard Dujon

Abstract

We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragments, representing the largest collection of toxic inserts published so far in yeast.

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Jun 23, 2012·Nature·Anne-Ruxandra CarvunisMarc Vidal
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Methods Mentioned

BETA
GTPases
ubiquitination
PCR
electrophoresis
two-hybrid

Software Mentioned

TopPredII
BLASTP
GPROTEOME3
Conserved Domain Search service
- Search
DOGEL
European Molecular Biology Open Software Suite ( EMBOSS )
MIPS
CD
Search

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