Large-scale gene expression analysis of human skeletal myoblast differentiation

Neuromuscular Disorders : NMD
Ellen SterrenburgJohan T den Dunnen

Abstract

To study pathways involved in human skeletal myogenesis, we profiled gene expression in human primary myoblast cells derived from three individuals using both oligonucleotide and cDNA microarrays. Following stringent statistical testing (false-positive rate 0.4%), we identified 146 genes differentially expressed over time. Interestingly, 86 of these genes have not been reported to be involved in myogenesis in mouse cell lines. This demonstrates the additional value of human primary cell cultures in the study of muscle differentiation. Many of the identified genes play a role in muscle regeneration, indicating the close relationship of this process with muscle development. In addition, we found overlap with expression profiling studies in muscle from Duchenne muscular dystrophy patients, confirming ongoing muscle regeneration in Duchenne muscular dystrophy. Further study of these genes can bring new insights into the process of muscle differentiation, and they are candidate genes for neuromuscular disorders with an as yet unidentified cause.

References

Sep 20, 1991·Cell·J M Ervasti, K P Campbell
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J H Miner, B Wold
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J MassaguéB Nadal-Ginard
Jan 7, 1998·Molecular and Cellular Biology·R J Wechsler-ReyaG C Prendergast
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z YanR S Williams
Jun 23, 1998·Current Biology : CB·H AmthorK Patel
Mar 25, 2000·Clinical Genetics·L A Sabourin, M A Rudnicki
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
May 23, 2002·The Journal of Biological Chemistry·Po ZhaoEric P Hoffman
May 29, 2002·Neuromuscular Disorders : NMD·Francesco MuntoniKetan Patel
Sep 11, 2002·Neuromuscular Disorders : NMD·Annemieke Aartsma-RusJudith C T van Deutekom
Nov 1, 2002·Nucleic Acids Research·Ellen SterrenburgJohan T den Dunnen
Nov 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Judith N HaslettLouis M Kunkel
Dec 13, 2002·The Journal of Biological Chemistry·Zhen YanR Sanders Williams
Feb 22, 2003·Nucleic Acids Research·Peter A C 't HoenJohan T den Dunnen
Mar 7, 2003·Human Molecular Genetics·Satoru NoguchiIchizo Nishino
Apr 2, 2003·Human Molecular Genetics·Annemieke Aartsma-RusJudith C T van Deutekom

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Citations

Jun 15, 2007·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Naftali Kaminski, Ziv Bar-Joseph
Jul 1, 2008·Bioinformatics·Tien-ho LinZiv Bar-Joseph
Oct 9, 2007·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Mark TarnopolskyRobert Isfort
Sep 29, 2011·BMC Medical Genomics·Koji TsumagariMelanie Ehrlich
Jul 23, 2009·PloS One·Amy C MaherMark A Tarnopolsky
Jun 7, 2014·In Vitro Cellular & Developmental Biology. Animal·Smritee PokharelInho Choi
May 2, 2009·Ontogenez·A A MakarovS S Shishkin
Oct 21, 2009·Journal of Cellular and Molecular Medicine·Elise JeanGilles Carnac
Jun 16, 2015·Journal of Proteome Research·Marie-Catherine Le BihanIrina Kratchmarova
Apr 16, 2015·Nucleic Acids Research·Eleonora de KlerkPeter A C 't Hoen
Apr 6, 2017·Bioinformatics·Sabrina RashidZiv Bar-Joseph
Sep 12, 2019·Cell Biology International·Yi-Tzu LinCorinne M Linardic
Mar 12, 2020·Journal of Cachexia, Sarcopenia and Muscle·Jessy EtienneJean-Thomas Vilquin
Jul 11, 2020·International Journal of Molecular Sciences·Anna CiecierskaTomasz Sadkowski

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