Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

Nature Protocols
R Daniel Gietz, Robert H Schiestl


Here, we describe a Library screen transformation protocol using the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae. This method is suitable for screening complex plasmid libraries such as those used for yeast two-hybrid analysis. This procedure takes up to 2.5 h to complete once the yeast culture has been grown.


Jan 1, 1987·Methods in Enzymology·M D Rose
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Apr 3, 2007·Nature Protocols·R Daniel Gietz, Robert H Schiestl

❮ Previous
Next ❯


Oct 13, 2011·Bioprocess and Biosystems Engineering·Dong Min KimIl-Chul Kim
Mar 27, 2013·Antonie van Leeuwenhoek·Petar Tomev Mitrikeski
Jun 23, 2012·Nature Protocols·Ryan HietpasDaniel N A Bolon
Jun 30, 2011·The Journal of Biological Chemistry·Yibing RuanFrans A van der Hoorn
Apr 5, 2012·The Journal of Biological Chemistry·Antje BrüserTorsten Schöneberg
Dec 13, 2006·FEMS Yeast Research·Paul J CullenGeorge F Sprague
Oct 5, 2010·Applied and Environmental Microbiology·David RunquistMaurizio Bettiga
Jun 9, 2011·BMC Evolutionary Biology·Yevgeniy RaynesPaul D Sniegowski
May 17, 2011·Microbial Cell Factories·William A Rodríguez-LimasLaura A Palomares
Jan 4, 2012·Biotechnology for Biofuels·André SchusterMonika Schmoll
Oct 5, 2010·RNA·J Michael Charette, Susan J Baserga
Jan 29, 2010·PLoS Genetics·Joshua A Granek, Paul M Magwene
Mar 23, 2012·PLoS Genetics·Dalia RosinNaama Barkai
Aug 24, 2012·PloS One·Samina Naseeb, Daniela Delneri
Jan 10, 2013·PloS One·Walter W Steiner, Estelle M Steiner
Jul 31, 2013·PloS One·Lisvane Silva PaesAriel Mariano Silber
Sep 17, 2013·PloS One·Basti BergdahlMarie F Gorwa-Grauslund

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.