Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

Nature Protocols
R Daniel Gietz, Robert H Schiestl

Abstract

Here, we describe a Library screen transformation protocol using the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae. This method is suitable for screening complex plasmid libraries such as those used for yeast two-hybrid analysis. This procedure takes up to 2.5 h to complete once the yeast culture has been grown.

References

Jan 1, 1987·Methods in Enzymology·M D Rose
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Apr 3, 2007·Nature Protocols·R Daniel Gietz, Robert H Schiestl

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Citations

Oct 13, 2011·Bioprocess and Biosystems Engineering·Dong Min KimIl-Chul Kim
Mar 27, 2013·Antonie van Leeuwenhoek·Petar Tomev Mitrikeski
Jun 23, 2012·Nature Protocols·Ryan HietpasDaniel N A Bolon
Jun 30, 2011·The Journal of Biological Chemistry·Yibing RuanFrans A van der Hoorn
Apr 5, 2012·The Journal of Biological Chemistry·Antje BrüserTorsten Schöneberg
Dec 13, 2006·FEMS Yeast Research·Paul J CullenGeorge F Sprague
Oct 5, 2010·Applied and Environmental Microbiology·David RunquistMaurizio Bettiga
Jun 9, 2011·BMC Evolutionary Biology·Yevgeniy RaynesPaul D Sniegowski
May 17, 2011·Microbial Cell Factories·William A Rodríguez-LimasLaura A Palomares
Jan 4, 2012·Biotechnology for Biofuels·André SchusterMonika Schmoll
Oct 5, 2010·RNA·J Michael Charette, Susan J Baserga
Jan 29, 2010·PLoS Genetics·Joshua A Granek, Paul M Magwene
Mar 23, 2012·PLoS Genetics·Dalia RosinNaama Barkai
Aug 24, 2012·PloS One·Samina Naseeb, Daniela Delneri
Jan 10, 2013·PloS One·Walter W Steiner, Estelle M Steiner
Jul 31, 2013·PloS One·Lisvane Silva PaesAriel Mariano Silber
Sep 17, 2013·PloS One·Basti BergdahlMarie F Gorwa-Grauslund

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