Large-scale identification of core-fucosylated glycopeptide sites in pancreatic cancer serum using mass spectrometry

Journal of Proteome Research
Zhijing TanDavid M Lubman

Abstract

Glycosylation has significant effects on protein function and cell metastasis, which are important in cancer progression. It is of great interest to identify site-specific glycosylation in search of potential cancer biomarkers. However, the abundance of glycopeptides is low compared to that of nonglycopeptides after trypsin digestion of serum samples, and the mass spectrometric signals of glycopeptides are often masked by coeluting nonglycopeptides due to low ionization efficiency. Selective enrichment of glycopeptides from complex serum samples is essential for mass spectrometry (MS)-based analysis. Herein, a strategy has been optimized using LCA enrichment to improve the identification of core-fucosylation (CF) sites in serum of pancreatic cancer patients. The optimized strategy was then applied to analyze CF glycopeptide sites in 13 sets of serum samples from pancreatic cancer, chronic pancreatitis, healthy controls, and a standard reference. In total, 630 core-fucosylation sites were identified from 322 CF proteins in pancreatic cancer patient serum using an Orbitrap Elite mass spectrometer. Further data analysis revealed that 8 CF peptides exhibited a significant difference between pancreatic cancer and other controls, whi...Continue Reading

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Citations

Oct 21, 2015·Drug Discovery Today·Matheus H DiasLeo K Iwai
Oct 28, 2015·Journal of Proteome Research·Yiwei ZhangDavid M Lubman
Sep 26, 2015·Journal of Proteome Research·Haidi YinDavid M Lubman
Sep 4, 2015·Carbohydrate Research·Feng GuanGanglong Yang
Sep 4, 2016·Analytical and Bioanalytical Chemistry·Muchena J KailemiaCarlito B Lebrilla
Oct 21, 2016·Electrophoresis·Alireza BanazadehYehia Mechref
Mar 1, 2017·Journal of Proteome Research·Anna DrabikMarek Sierzega
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Jul 29, 2017·Electrophoresis·Mengmeng WangChunfang Gao
Nov 10, 2017·Mass Spectrometry Reviews·Robert MoulderRiitta Lahesmaa
Jan 22, 2018·Analytical and Bioanalytical Chemistry·Ana Díaz-FernándezMaría Jesús Lobo-Castañón
Sep 13, 2017·PeerJ·Onn Haji HashimCheng-Siang Lee
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