Large-scale mutagenesis and phenotypic screens for the nervous system and behavior in mice

Trends in Neurosciences
Martha Hotz VitaternaJoseph S Takahashi

Abstract

Significant developments have occurred in our understanding of the mammalian genome thanks to informatics, expression profiling and sequencing of the human and rodent genomes. However, although these facets of genomic analysis are being addressed, analysis of in vivo gene function remains a formidable task. Evaluation of the phenotype of mutants provides powerful access to gene function, and this approach is particularly relevant to the nervous system and behavior. Here, we discuss the complementary mouse genetic approaches of gene-driven, targeted mutagenesis and phenotype-driven, chemical mutagenesis. We highlight an NIH-supported large-scale effort to use phenotype-driven mutagenesis screens to identify mouse mutants with neural and behavioral alterations. Such single-gene mutations can then be used for gene identification using positional candidate gene-cloning methods.

References

Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·R J Konopka, S Benzer
Oct 30, 1980·Nature·C Nüsslein-Volhard, E Wieschaus
Mar 1, 1995·Nature Medicine·K Paigen
Apr 1, 1995·Nature Genetics·F S Collins
Jun 17, 1994·Science·J S TakahashiM H Vitaterna
Jan 1, 1994·Annual Review of Neuroscience·S L Zipursky, G M Rubin
Jan 1, 1993·Annual Review of Physiology·C S Pittendrigh
Mar 14, 1996·Nature·W F DietrichT J O'Connor
Nov 1, 1996·Trends in Genetics : TIG·S D Brown, J Peters
Feb 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·S SignoriniM Stoffel
May 16, 1997·Cell·D P KingJ S Takahashi
Oct 8, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·D C RogersJ E Martin
Oct 23, 1998·Science·F S CollinsL Walters
Jan 23, 1999·Nature Genetics·R J LipshutzD J Lockhart
Jan 23, 1999·Nature Genetics·P O Brown, D Botstein
Apr 18, 2000·Human Molecular Genetics·L M Tarantino, M Bucan
Aug 10, 2000·Nature Genetics·M H Hrabé de AngelisR Balling
Mar 10, 2001·Science·J H NadeauUNKNOWN International Mouse Mutagenesis Consortium
Mar 10, 2001·Nature·E S LanderUNKNOWN International Human Genome Sequencing Consortium
Jul 27, 2001·Mammalian Genome : Official Journal of the International Mammalian Genome Society·S O MoldinJ F Battey
Dec 6, 2001·Trends in Molecular Medicine·D C Bullard
Jan 31, 2002·Nature Neuroscience
Feb 12, 2002·Nature Reviews. Genetics·Maja Bućan, Ted Abel
Jun 25, 2002·Trends in Neurosciences·David P WolferHans Peter Lipp
Aug 16, 2002·Nature·Simon G GregoryDavid R Bentley
Oct 2, 2002·FEBS Letters·François TroncheChristoph Kellendonk
Oct 4, 2002·Biological Psychiatry·Kathleen R MerikangasJoseph S Takahashi
Nov 14, 2002·Annual Review of Genetics·Anna MatyniaAlcino J Silva
Dec 6, 2002·Nature·Allan Bradley
Dec 6, 2002·Nature·UNKNOWN Mouse Genome Sequencing ConsortiumEric S Lander
Dec 6, 2002·Nature·Y OkazakiUNKNOWN RIKEN Genome Exploration Research Group Phase I & II Team
Dec 6, 2002·Nature·Claire M WadeMark J Daly
Jan 17, 2003·Genome Research·Kelly A FrazerRoss C Hardison
Mar 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Tim WiltshireColin F Fletcher
Apr 3, 2003·Nature Reviews. Genetics·Abel Ureta-VidalEwan Birney
May 20, 2003·Human Mutation·Peter D StensonDavid N Cooper
Jul 29, 2003·Genes, Brain, and Behavior·A EastonF W Turek
Apr 2, 2004·Nature·Richard A GibbsUNKNOWN Rat Genome Sequencing Project Consortium

❮ Previous
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Citations

Apr 14, 2007·Visual Neuroscience·Lawrence H PintoJoseph S Takahashi
Apr 19, 2008·Cold Spring Harbor Symposia on Quantitative Biology·Sandra M SiepkaJoseph S Takahashi
Nov 8, 2008·Science·Joseph S TakahashiVivek Kumar
Sep 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Vivek KumarJoseph S Takahashi
Jul 17, 2007·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Melloni N CookDan Goldowitz
Jan 20, 2012·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Jeffrey WeissJ Larry Jameson
Jun 2, 2012·Expert Opinion on Drug Discovery·Adam StewartAllan V Kalueff
Dec 5, 2006·Trends in Endocrinology and Metabolism : TEM·Akira Kohsaka, Joseph Bass
Dec 18, 2013·Annals of the New York Academy of Sciences·Ellen FrankBrant P Hasler
Sep 11, 2007·Vision Research·Stewart ThompsonEdwin M Stone
Aug 30, 2008·Trends in Pharmacological Sciences·André Ramos
Feb 11, 2014·Behavioural Brain Research·Allan V Kalueff, Adam Michael Stewart
May 10, 2017·Human Molecular Genetics·Kacie J Meyer, Michael G Anderson
May 15, 2007·Pharmacogenetics and Genomics·Corinne RenierEmmanuel Mignot
May 7, 2019·Genes, Brain, and Behavior·Marleen H M de GrootJoseph S Takahashi
Jun 10, 2020·Experimental Biology and Medicine·Wei-Hua LeeAkihiro Ikeda
Sep 1, 2017·PloS One·Mark P KrebsPatsy M Nishina
May 3, 2019·Scientific Reports·Adam Hedberg-BuenzMichael G Anderson

❮ Previous
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