Large-scale patterning of living colloids for dynamic studies of neutrophil-microbe interactions

Lab on a Chip
Jae Jung KimDaniel Irimia

Abstract

Neutrophils are the first white blood cells to respond to microbes and to limit their invasion of the body. However, the growth of the microbes into colonies often challenges the neutrophils ability to contain them. To study the interactions between neutrophils and microbial colonies, we designed an assay for arranging microbes in clusters of controlled size (i.e. living colloids). The patterned microbes in the living colloid are mechanically trapped inside the wells and fully accessible to neutrophils. Using the assay, we studied the interactions between human neutrophils and Candida albicans and Staphylococcus aureus, two common human pathogens. We also probed the susceptibility of C. albicans colloids to caspofungin, a common antifungal drug.

References

Jul 27, 2002·Analytical Chemistry·Israel Biran, David R Walt
Apr 4, 2003·Analytical Biochemistry·Israel BiranDavid R Walt
Jan 6, 2004·Biotechnology and Bioengineering·Tao XuThomas Boland
May 18, 2004·Analytical Chemistry·Yina KuangDavid R Walt
May 25, 2005·Annals of Biomedical Engineering·Francis LinNoo Li Jeon
Sep 1, 2005·Analytical Chemistry·Jacqueline R Rettig, Albert Folch
Sep 3, 2005·Nature Reviews. Microbiology·Søren J SørensenStefan Wuertz
Jan 21, 2006·Journal of Immunological Methods·N R ThirumalapuraJ R Malayer
Feb 17, 2007·Nature Reviews. Microbiology·Burkhard A HenseJan-Ulrich Kreft
Nov 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Colin J InghamWillem M de Vos
Mar 28, 2008·Biotechnology and Bioengineering·James D Bryers
Jun 17, 2008·Colloids and Surfaces. B, Biointerfaces·Aline CerfChristophe Vieu
Nov 17, 2009·Nature Methods·Holger KressEric R Dufresne
Mar 30, 2010·Nature Reviews. Microbiology·Anton Y PelegEleftherios Mylonakis
Jul 29, 2010·Physiological Reviews·Inna SekirovB Brett Finlay
Aug 3, 2010·Tissue Engineering. Part C, Methods·Yu-Suke TorisawaShuichi Takayama
Oct 7, 2010·Proceedings of the National Academy of Sciences of the United States of America·Daniel KreiselMark J Miller
Mar 17, 2011·Nature Reviews. Microbiology·Elizabeth A Grice, Julia A Segre
May 6, 2011·PloS One·Anand SrinivasanAnand K Ramasubramanian
Jun 24, 2011·The Journal of Investigative Dermatology·Lai Guan NgWolfgang Weninger
Sep 17, 2011·Virulence·Nathan K ArcherMark E Shirtliff
Dec 14, 2011·International Journal of Microbiology·Hélène Tournu, Patrick Van Dijck
Jun 16, 2012·Nature·UNKNOWN Human Microbiome Project Consortium
Sep 14, 2012·Nature·Catherine A LozuponeRob Knight
Dec 21, 2012·Science Translational Medicine·Gordon D BrownTheodore C White
May 28, 2013·Nature·Tim LämmermannRonald N Germain
Jun 27, 2013·MBio·Anand SrinivasanAnand K Ramasubramanian
Nov 26, 2013·Seminars in Immunology·James A Sanford, Richard L Gallo
Dec 24, 2013·International Journal of Antimicrobial Agents·Gordon RamageCraig Williams
Aug 26, 2014·Folia Microbiologica·Jinzhi HeXuedong Zhou
Sep 4, 2014·Nature Communications·Leo BoneschanskerDaniel Irimia
Dec 19, 2014·Science Translational Medicine·Jungil ChoiSunghoon Kwon
Aug 15, 2015·The Journal of Infectious Diseases·Caroline N JonesDaniel Irimia
Sep 10, 2015·Microbiology Spectrum·Jyotsna Chandra, Pranab K Mukherjee
Oct 22, 2015·Annual Review of Microbiology·Clarissa J Nobile, Alexander D Johnson
Nov 26, 2015·Biomicrofluidics·Collin M TimmDale A Pelletier
Dec 15, 2015·Journal of Chromatographic Science·Rui LiYulin Deng
Aug 26, 2016·Immunological Reviews·Korbinian Kienle, Tim Lämmermann
Nov 1, 2016·Nature Materials·Jae Jung KimPatrick S Doyle
Mar 16, 2017·Antimicrobial Agents and Chemotherapy·Matthew B LohseClarissa J Nobile

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Citations

Feb 26, 2019·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Mark A RoseStephen A Morin
Jan 30, 2021·Advanced Healthcare Materials·Meilin SunWenming Liu

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Methods Mentioned

BETA
fluorescence microscopy
confocal microscopy
Profiler
ELISA

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