Large-scale patterning of single cells and cell clusters in hydrogels

Scientific Reports
Xiangyu Gong, Kristen L Mills

Abstract

Biophysical properties of the extracellular matrix (ECM) are known to play a significant role in cell behavior. To gain a better understanding of the effects of the biophysical microenvironment on cell behavior, the practical challenge is longitudinally monitoring behavioral variations within a population to make statistically powerful assessments. Population-level measurements mask heterogeneity in cell responses, and large-scale individual cell measurements are often performed in a one-time, snapshot manner after removing cells from their matrix. Here we present an easy and low-cost method for large-scale, longitudinal studies of heterogeneous cell behavior in 3D hydrogel matrices. Using a platform we term "the drop-patterning chip", thousands of cells were simultaneously transferred from microwell arrays and fully embedded, only using the force of gravity, in precise patterns in 3D collagen I or Matrigel. This method allows for throughputs approaching 2D patterning methods that lack phenotypic information on cell-matrix interactions, and does not rely on special equipment and cell treatments that may result in a proximal stiff surface. With a large and yet well-organized group of cells captured in 3D matrices, we demonstrate...Continue Reading

References

Apr 29, 1994·Science·R SinghviD E Ingber
Nov 27, 2001·Science·E CukiermanK M Yamada
Jun 17, 2004·Biosensors & Bioelectronics·Darren S GrayChristopher S Chen
Dec 24, 2004·Lab on a Chip·Dirk R AlbrechtSangeeta N Bhatia
Sep 1, 2005·Analytical Chemistry·Jacqueline R Rettig, Albert Folch
Apr 22, 2006·Nature Methods·Dirk R AlbrechtSangeeta N Bhatia
Oct 27, 2006·Lab on a Chip·Dino Di CarloLuke P Lee
Apr 17, 2007·Biomaterials·Adam RosenthalJoel Voldman
May 15, 2007·Nature·Manuel ThéryFrank Jülicher
Jan 24, 2009·Nature Reviews. Cancer·Darci T ButcherValerie M Weaver
Mar 3, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Kirsty LeongAlex K-Y Jen
Apr 4, 2009·Biomedical Microdevices·Ulrike HaesslerMingming Wu
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Matthias P LutolfHelen M Blau
Jan 26, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Melissa G MendezRobert D Goldman
Aug 24, 2010·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Myong-Hee Sung, James G McNally
Oct 23, 2010·The Analyst·Yi-Chung TungShuichi Takayama
Dec 3, 2010·Journal of Cell Science·Christian FrantzValerie M Weaver
Mar 15, 2011·Advanced Materials·Franziska KleinMartin Bastmeyer
Jun 21, 2011·Small GTPases·Johanna Ivaska
Aug 25, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Jie PanChris Abell
May 31, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ying ZhengAbraham D Stroock
Jul 12, 2012·Nature Biotechnology·Sangeeta BhatiaKornelia Polyak
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Mar 19, 2013·Inflammatory Bowel Diseases·Laura A JohnsonPeter D Higgins
Feb 12, 2014·Proceedings of the National Academy of Sciences of the United States of America·Kai ZhangLidong Qin
Dec 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·Feng GuoTony Jun Huang
Jan 28, 2015·Scientific Reports·Akio SoedaDeric M Park
Jun 11, 2015·Lab on a Chip·Ching-Hui LinChia-Hsien Hsu
Sep 1, 2015·Nature Methods·Michael E TodhunterZev J Gartner
Jan 6, 2016·Biomaterials·Girdhari Rijal, Weimin Li
Feb 12, 2017·Scientific Reports·Shawn P CareyCynthia A Reinhart-King

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Citations

Jun 6, 2019·Advanced Materials·Sarvesh Kumar SrivastavaAnja Boisen
Mar 4, 2020·Proceedings of the National Academy of Sciences of the United States of America·Susan E LeggettIan Y Wong

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Methods Mentioned

BETA
chip
chips
confocal microscopy
Fluorescence
Fluorescence imaging

Software Mentioned

SolidWorks
Imaris
ZEN

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