Large-scale pre-diagnosis study of fetal RHD genotyping by PCR on plasma DNA from RhD-negative pregnant women

Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology
Christelle Rouillac-Le SciellourYves Brossard

Abstract

The routine prenatal determination of fetal RhD blood group would be very useful in the management of pregnancies in RhD-negative women, as up to 40% of these pregnancies bear a RhD-negative fetus. The fetal DNA present in maternal plasma offers an opportunity for risk-free prenatal diagnosis. This study focused on the feasibility and accuracy of large-scale RhD fetal diagnosis in non-immunized and anti-D immunized RhD-negative women. Plasma DNA was extracted from 893 RhD-negative pregnant women and amplified in exons 7 and 10 of the RHD gene using conventional and real-time PCR. The results were then compared with the RHD fetal genotype determined on amniotic cells and/or the RhD phenotype of the red blood cells of the infants at birth. After exclusion of 42 samples from women exhibiting a nonfunctional or rearranged RHD gene, fetal RhD status was predicted with a 99.5% accuracy. A strategy is also proposed to avoid the small number of false-positive and -negative results. Fetal RHD genotyping from maternal plasma DNA in different clinical situations may be used with confidence.

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References

Dec 1, 1983·American Journal of Medical Genetics·J C MurrayD A Luthy
Jan 1, 1994·Vox Sanguinis·T BluntB Carritt
Aug 26, 1993·The New England Journal of Medicine·P R BennettJ P Cartron
Aug 1, 1997·British Journal of Haematology·J T AubinJ P Cartron
Aug 16, 1997·Lancet·Y M LoJ S Wainscoat
Oct 20, 1998·Lancet·B H FaasC E van der Schoot
Dec 5, 1998·Transfusion·P A Maaskant-van WijkC E van der Schoot
Dec 10, 1998·The New England Journal of Medicine·Y M LoJ S Wainscoat
Mar 18, 2000·Journal of Human Genetics·H Cao, R A Hegele
May 3, 2000·Seminars in Hematology·C H HuangJ G Cheng
May 17, 2000·Current Opinion in Obstetrics & Gynecology·R D Wilson
May 20, 2000·Annals of the New York Academy of Sciences·J ZhangY M Lo
Jun 10, 2000·BJOG : an International Journal of Obstetrics and Gynaecology·X Y ZhongS Hahn
Aug 15, 2000·Seminars in Hematology·J B Bussel
Aug 10, 2001·BMC Genetics·F F WagnerW A Flegel
Oct 3, 2002·British Journal of Haematology·Jean-Marc CostaEvelyne Gautier
Jan 2, 2003·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Tobias J LeglerMichael Köhler
Jan 11, 2003·Transfusion Medicine Reviews·C Ellen van der SchootGodelieve C M L Christiaens
Nov 25, 2003·Vox Sanguinis·I RandenM K Fagerhol

❮ Previous
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Citations

May 21, 2005·Human Genetics·Silvia GalbiatiLaura Cremonesi
Jun 18, 2005·Blood Reviews·Caroline Le Van KimJean-Pierre Cartron
Oct 21, 2006·Current Opinion in Hematology·Connie M Westhoff
May 21, 2010·Clinical Chemistry and Laboratory Medicine : CCLM·Leyre CardoFrancisco V Alvarez
Feb 5, 2008·Gynécologie, obstétrique & fertilité·B CarbonneY Brossard
Oct 4, 2005·Obstetrics and Gynecology·Diana W BianchiC Ellen van der Schoot
Apr 9, 2008·BMJ : British Medical Journal·Sailesh Kumar
Apr 8, 2010·Expert Review of Molecular Diagnostics·Aggeliki KolialexiAriadni Mavrou
Nov 13, 2008·Expert Review of Molecular Diagnostics·Ossie Geifman-Holtzman, Janet Ober Berman
Sep 15, 2015·Expert Review of Molecular Diagnostics·Annika M Svensson, Meghan Delaney
Jun 2, 2010·Clinics in Laboratory Medicine·Geoff DanielsPete Martin
Mar 9, 2010·Gynécologie, obstétrique & fertilité·B CarbonneY Brossard
May 8, 2009·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·L Mannessier
Dec 17, 2008·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Karoline FreemanLeeza Osipenko
Apr 1, 2008·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·C Rouillac-Le SciellourJ-P Cartron
Feb 6, 2008·Seminars in Fetal & Neonatal Medicine·C Ellen van der SchootLyn S Chitty
May 22, 2007·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·Neil D Avent
Oct 19, 2005·Prenatal Diagnosis·Frederik Banch ClausenMorten Hanefeld Dziegiel
Jun 24, 2009·European Journal of Clinical Investigation·X D WangZ M He
Nov 28, 2007·Transfusion·Jean-Marc MinonJean-Michel Foidart
Nov 20, 2008·British Journal of Haematology·Neil D Avent
Jul 31, 2007·Transfusion·Gregory A Denomme, Bernard J Fernandes
Jun 15, 2011·BJOG : an International Journal of Obstetrics and Gynaecology·P G SchefferM de Haas
Mar 28, 2006·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·W A Flegel
Jul 26, 2005·Seminars in Hematology·Kenneth J Moise
Sep 13, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yan Yun Wu, Gyorgy Csako
Aug 17, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Gyorgy Csako
Sep 27, 2006·American Journal of Obstetrics and Gynecology·Ossie Geifman-HoltzmanJohn P Gaughan
Mar 5, 2005·American Journal of Obstetrics and Gynecology·Evelyne GautierJean-Marc Costa
Mar 20, 2014·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Frederik Banch ClausenMorten Hanefeld Dziegiel
Mar 28, 2006·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·C E Van der SchootM de Haas

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