Large-Scale Preparation of Giant Vesicles by Squeezing a Lipid-Coated Marshmallow-like Silicone Gel in a Buffer

Langmuir : the ACS Journal of Surfaces and Colloids
Gen Hayase, Shin-Ichiro M Nomura

Abstract

Giant vesicles were efficiently produced by squeezing a lipid (l-α-phosphatidylcholine from egg yolk)-coated marshmallow-like flexible macroporous silicone monolith in a buffer. The mean diameter of the obtained vesicles was 2 μm, showing a wide distribution, up to tens of micrometers, which was similar to that of vesicles formed by a natural swelling method. It was possible to prepare vesicle dispersions on a scale from several microliters to several hundred milliliters. A protein synthesis system (PURE system) contained in vesicles prepared using this method functioned effectively. Our absorbing-squeezing method is expected to help in studies that use giant vesicles such as artificial cells and drug delivery systems.

References

Jan 30, 1991·Biochimica Et Biophysica Acta·R C MacDonaldL R Hu
Jan 1, 1980·Annual Review of Biophysics and Bioengineering·F Szoka, D Papahadjopoulos
Jul 1, 1997·Nature Biotechnology·N S TempletonG N Pavlakis
Dec 15, 1991·Physical Review. a·M Kummrow, W Helfrich
Aug 2, 2001·Nature Biotechnology·Y ShimizuT Ueda
Sep 29, 2001·Biomolecular Engineering·P Walde, S Ichikawa
Jan 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ann-Sofie CansAndrew Ewing
Nov 13, 2003·Chembiochem : a European Journal of Chemical Biology·Shin-ichiro M NomuraKenichi Yoshikawa
Aug 23, 2006·Molecular Systems Biology·Anthony C Forster, George M Church
Apr 25, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Andreas JahnMichael Gaitan
Jul 11, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Ho Cheung ShumDavid A Weitz
Apr 29, 2009·Chemistry and Physics of Lipids·Tsuyoshi YamaguchiShinobu Koda
Mar 26, 2010·Chembiochem : a European Journal of Chemical Biology·Peter WaldePasquale Stano
Jul 19, 2008·Annual Review of Analytical Chemistry·Aldo Jesorka, Owe Orwar
May 15, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Koji NishimuraTetsuya Yomo
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Jul 26, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Susanne F Fenz, Kheya Sengupta
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Jan 18, 2013·PloS One·Kei Fujiwara, Shin-ichiro M Nomura
Feb 26, 2013·Nanoscale Research Letters·Abolfazl AkbarzadehKazem Nejati-Koshki
Jul 5, 2013·Biophysical Journal·Andreas WeinbergerCarlos Marques
Nov 14, 2013·Chemistry and Physics of Lipids·Yogita P Patil, Sameer Jadhav
Dec 18, 2013·Journal of Pharmaceutical Sciences·John C KraftRodney J Y Ho
Jul 14, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Yasmine Miguel Serafini MichelettoAndré P Schroder
Oct 25, 2017·Synthetic and Systems Biotechnology·Filippo Caschera
Dec 1, 2017·BMC Bioinformatics·Curtis T RuedenKevin W Eliceiri
Mar 1, 2017·Science Robotics·Yusuke SatoShin-Ichiro M Nomura

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.