PMID: 40881Oct 1, 1979

Large-scale production and physicochemical characterization of human immune interferon

Infection and Immunity
M P LangfordH M Johnson

Abstract

Large-scale production of crude high-titered (10(2.3) to 10(4) U/ml) human immune interferon (type II) was carried out in roller bottle cultures of human peripheral lymphocytes by using the T-cell mitogen staphylococcal enterotoxin A. Over 99% of human immune interferon was destroyed by pH 2 or heat at 56 degrees C for 1 h. The interferon was not neutralized by antibody to human leukocyte interferon. The kinetics of development of the antiviral state were slow for immune interferon relative to those for leukocyte interferon. Ultrogel AcA 54 chromatography of crude or the concentrated interferon resulted in two peaks of activity, a major one (87% of recovered activity) with a molecular weight of 40,000 to 46,000 and a minor peak of molecular weight 65,000 to 70,000. The column elution buffer consisting of 18% ethylene glycol and 1 M NaCl in phosphate-buffered saline resulted in at least 100% recovery of added interferon. The data suggest, then, that the interferon produced under large-scale conditions was immune (type II). The efficiency of the production was comparable to that described for large-scale production of human leukocyte interferon. Our large-scale production system for human immune interferon offers a feasible appro...Continue Reading

References

Oct 13, 1992·Annals of the New York Academy of Sciences·E I TsaoM J Munster
Apr 1, 1987·Immunological Investigations·M P LangfordJ A Grant
Apr 1, 1985·International Journal of Dermatology·J L JorizzoK M Fagan
Jan 1, 1984·Journal of Interferon Research·S Takano, Y Nagano
Oct 1, 1984·European Journal of Immunology·P W AndrewD B Lowrie
Jan 1, 1988·Journal of Veterinary Internal Medicine·K L Jacobsen, G A Rockwood
Oct 1, 1990·Immunological Investigations·M P LangfordR Kaiwar
Jun 1, 1991·FEMS Microbiology Immunology·A C WoodJ P Arbuthnott
Jan 1, 1986·Preparative Biochemistry·M YasudaN K Day
May 2, 1986·European Journal of Biochemistry·J H MutsaersJ F Vliegenthart
Oct 1, 1986·Journal of Interferon Research·I RosztóczyI Béládi
Jan 1, 1981·Journal of Interferon Research·R E McCoolT L Ratliff
Jan 1, 1984·Critical Reviews in Clinical Laboratory Sciences·W J Richtsmeier
Jul 1, 1987·European Journal of Biochemistry·Y C PanR Chizzonite
Sep 24, 1982·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·W FiersJ Content
Feb 15, 1981·International Journal of Cancer. Journal International Du Cancer·C A BironJ S Pagano

Related Concepts

Buffers
T-Lymphocyte
IFNG
Ethylene Glycol Measurement
Staphylococcal enterotoxin
Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Chromatography
Lymphoid Cells
Human leukocyte interferon
Phosphate Measurement

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.