Large-scale profiling of diterpenoid glycosides from Stevia rebaudiana using ultrahigh performance liquid chromatography/tandem mass spectrometry

Analytical and Bioanalytical Chemistry
Behnaz ShafiiA Daniel Jones

Abstract

The plant Stevia rebaudiana accumulates a suite of diterpenoid metabolites that are natural sweeteners finding increased use as sugar substitutes. To guide breeding of stevia plants that accumulate substances with desirable flavor in high yield, rapid and accurate methods are needed to profile these substances in plant populations. This report describes an 8-min ultrahigh performance liquid chromatography-tandem mass spectrometry method for separation and quantification of seven stevia glycosides including steviolbioside; stevioside; rebaudiosides A, B, and C; rubusoside; and dulcoside as well as aglycones steviol and isosteviol. This negative mode electrospray ionization/multiple reaction monitoring method yielded low limits of detection <1 ng/mL for steviol, 6 ng/mL for isosteviol, and <15 ng/mL for all stevia glycosides. Stevioside and Reb A, B, and C were quantified in more than 1,100 extracts from stevia leaves as part of a large-scale profiling exercise. Leaf tissue levels in this population spanned about two orders of magnitude for stevioside (2-125 mg/g dry weight), Reb A (2.5-164 mg/g), Reb B (0.5-50 mg/g), and Reb C (1.5-125 mg/g), but levels of individual metabolites exhibited independent variation. The wide spread o...Continue Reading

References

Oct 16, 2001·Journal of Agricultural and Food Chemistry·N KolbR F Uliana
Dec 31, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·E KoyamaM Ui
Apr 29, 2004·Journal of Agricultural and Food Chemistry·Veerle J Y MinneJan M C Geuns
Jun 20, 2007·Nature Chemical Biology·Michael A Fischbach, Jon Clardy
Jul 13, 2007·Journal of Biotechnology·Brás H de OliveiraDaniel A de Jesus
Nov 26, 2009·The Plant Journal : for Cell and Molecular Biology·Liping GuRobert L Last
Feb 2, 2010·The Plant Journal : for Cell and Molecular Biology·Anthony SchilmillerRobert L Last
Nov 12, 2010·Journal of Agricultural and Food Chemistry·Ursula Wölwer-RieckAndreas Wawrzun

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Citations

Jan 24, 2013·Analytical and Bioanalytical Chemistry·Rahul S PawarJeanne I Rader
May 30, 2013·Journal of Natural Products·Stijn Ceunen, Jan M C Geuns
Dec 4, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Meiyu WangHongjian Zhang
Aug 15, 2013·Natural Product Reports·James R Hanson
Apr 25, 2020·Analytical and Bioanalytical Chemistry·Gertrud E Morlock, Julia Heil
Mar 15, 2018·Analytical Chemistry·Michael McCullaghSeverine Goscinny

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