Large scale RNAi screen in Tribolium reveals novel target genes for pest control and the proteasome as prime target

BMC Genomics
Julia UlrichGregor Bucher

Abstract

Insect pest control is challenged by insecticide resistance and negative impact on ecology and health. One promising pest specific alternative is the generation of transgenic plants, which express double stranded RNAs targeting essential genes of a pest species. Upon feeding, the dsRNA induces gene silencing in the pest resulting in its death. However, the identification of efficient RNAi target genes remains a major challenge as genomic tools and breeding capacity is limited in most pest insects impeding whole-animal-high-throughput-screening. We use the red flour beetle Tribolium castaneum as a screening platform in order to identify the most efficient RNAi target genes. From about 5,000 randomly screened genes of the iBeetle RNAi screen we identify 11 novel and highly efficient RNAi targets. Our data allowed us to determine GO term combinations that are predictive for efficient RNAi target genes with proteasomal genes being most predictive. Finally, we show that RNAi target genes do not appear to act synergistically and that protein sequence conservation does not correlate with the number of potential off target sites. Our results will aid the identification of RNAi target genes in many pest species by providing a manageable...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1997·Nucleic Acids Research·W M GelbartC Tolstoshev
Dec 10, 1999·Nature·A J BerghammerE A Wimmer
Jun 26, 2001·Science·A S Moffat
Feb 13, 2002·Current Biology : CB·Gregor BucherMartin Klingler
Sep 15, 2004·Development Genes and Evolution·Yoshinori Tomoyasu, Robin E Denell
Sep 17, 2004·Nature·Gunter Meister, Thomas Tuschl
Sep 12, 2006·Nature Methods·Meghana M KulkarniBernard Mathey-Prevot
Nov 6, 2007·Nature Biotechnology·James A BaumJames Roberts
Mar 26, 2008·Nature·UNKNOWN Tribolium Genome Sequencing ConsortiumGregor Bucher
May 3, 2008·Trends in Genetics : TIG·Jennifer S Whangbo, Craig P Hunter
May 27, 2008·Trends in Biotechnology·Daniel R G Price, John A Gatehouse
Jul 30, 2008·Development Genes and Evolution·Sherry C MillerYoshinori Tomoyasu
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Oct 10, 2009·Insect Biochemistry and Molecular Biology·Steven WhyardSylvia Wong
Feb 12, 2010·Cold Spring Harbor Protocols·Nico PosnienGregor Bucher
May 21, 2010·BMC Developmental Biology·Johannes B SchinkoGregor Bucher
Aug 15, 2012·Development Genes and Evolution·Johannes Benno SchinkoGregor Bucher
Jun 12, 2013·Nature Biotechnology·Bruce E TabashnikYves Carrière
Aug 3, 2013·Genetics·Richard H Ffrench-Constant
Sep 18, 2013·Journal of Insect Physiology·Jeffrey G ScottAngela E Douglas
Jul 9, 2014·Genome Biology·Richard H Ffrench-Constant
Nov 8, 2014·Nucleic Acids Research·Jürgen DönitzGregor Bucher
Jul 29, 2015·Nature Communications·Christian Schmitt-EngelGregor Bucher

❮ Previous
Next ❯

Citations

Mar 15, 2016·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Linda BingsohnAndreas Vilcinskas
May 25, 2016·Pest Management Science·Elane FishilevichBlair D Siegfried
Oct 27, 2017·Nucleic Acids Research·Jürgen DönitzGregor Bucher
Jun 20, 2017·Pest Management Science·Richard M Wilkins
Dec 5, 2017·Entomologia Experimentalis Et Applicata·Michael DarringtonTracey Chapman
Aug 17, 2019·Pest Management Science·Nitish KunteLuz-Adriana Avila
Dec 14, 2019·Archives of Insect Biochemistry and Physiology·Maria RigaJohn Vontas
Sep 11, 2019·Pest Management Science·Md Abdullah Al BakiYonggyun Kim
Nov 22, 2019·Neotropical Entomology·N P DiasM J Zotti
Apr 17, 2020·Archives of Insect Biochemistry and Physiology·Ramesh Kumar DhandapaniSubba Reddy Palli
Sep 8, 2017·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·B Mamta, M V Rajam
Aug 5, 2017·Frontiers in Molecular Neuroscience·Nina HahnRalf Heinrich
Jan 16, 2020·BMC Genomics·Nicolae HerndonGregor Bucher
Apr 30, 2020·Archives of Insect Biochemistry and Physiology·Jinmo KooSubba R Palli
Mar 3, 2020·Frontiers in Plant Science·Stephen J FletcherNeena Mitter
May 1, 2020·Archives of Insect Biochemistry and Physiology·Wesley P F MáximoSubba R Palli
Nov 5, 2019·Biotechnology Advances·Shaoshuai LiuKarl-Heinz Kogel
Jan 14, 2021·Pest Management Science·Kristopher SilverKun Yan Zhu
Feb 13, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Yuning HuLei Xu
Apr 1, 2018·Current Opinion in Insect Science·Duane D McKenna

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic

Software Mentioned

iBeetle
DAVID
iBeetle Base
Blast
Flybase Query Builder

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved