Large-scale separation of alkaloids from Corydalis decumbens by pH-zone-refining counter-current chromatography

Journal of Chromatography. a
Xiao WangJianhua Liu

Abstract

pH-zone-refining counter-current chromatography was successfully applied to the separation of alkaloids from a crude extract of Corydalis decumbens (Thunb.) Pers. using a multilayer coil planet centrifuge (CPC). The experiment was performed with a two-phase solvent system composed of methyl tert-butyl ether (MtBE)-acetonitrile-water (2:2:3, v/v) where triethylamine (5-10 mM) was added to the upper organic stationary phase as a retainer and hydrochloric acid (5-10 mM) to the aqueous mobile phase as an eluter. From 3.1 g of the crude extract, 495 mg protopine, 626 mg tetrahydropalmatine and 423 mg bicuculline were obtained each with a purity of over 93% as determined by high performance liquid chromatography. The structures of the isolated compounds were identified by electron ionization mass spectrometry (EI-MS), high-performance liquid chromatography (HPLC)-electrospray ionisation-mass spectrometry (ESI-MS) and 1H NMR.

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Citations

Jan 11, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xikai ShuBingtian Yang
May 24, 2008·Natural Product Reports·Otto Sticher
Jun 27, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qinghai ZhangAiqin Luo
Oct 14, 2014·Journal of Liquid Chromatography & Related Technologies·Dongyu GuYoichiro Ito
May 27, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anupam Maurya, Santosh Kumar Srivastava
Sep 8, 2011·Journal of Separation Science·Lei FangLuqi Huang
May 8, 2014·The New Phytologist·Yang NiuHang Sun
Nov 16, 2010·Critical Reviews in Biotechnology·C M AjilaJ R Valéro
Jun 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lu JiangWei Zhang
Mar 23, 2012·Journal of Chromatography. a·Leslie BoudesocqueJean-Hugues Renault

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