Large-scale topographical screen for investigation of physical neural-guidance cues

Scientific Reports
Wei LiStella W Pang

Abstract

A combinatorial approach was used to present primary neurons with a large library of topographical features in the form of micropatterned substrate for high-throughput screening of physical neural-guidance cues that can effectively promote different aspects of neuronal development, including axon and dendritic outgrowth. Notably, the neuronal-guidance capability of specific features was automatically identified using a customized image processing software, thus significantly increasing the screening throughput with minimal subjective bias. Our results indicate that the anisotropic topographies promote axonal and in some cases dendritic extension relative to the isotropic topographies, while dendritic branching showed preference to plain substrates over the microscale features. The results from this work can be readily applied towards engineering novel biomaterials with precise surface topography that can serve as guidance conduits for neuro-regenerative applications. This novel topographical screening strategy combined with the automated processing capability can also be used for high-throughput screening of chemical or genetic regulatory factors in primary neurons.

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Citations

Aug 19, 2015·Frontiers in Cellular Neuroscience·Devrim KilincGil U Lee
Oct 25, 2016·Scientific Reports·Zsófia BércesAnita Pongrácz
Apr 25, 2017·Nano Letters·Vini GautamVincent R Daria
Apr 7, 2018·Biomaterials Science·Chara SimitziEmmanuel Stratakis
Dec 21, 2017·BMC Biotechnology·Jacob J MessnerDeirdre R Meldrum
Sep 30, 2018·Nature Communications·Laurent PieuchotKarine Anselme
Dec 9, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chun-Yi YangTzu-Wei Wang
Dec 19, 2020·Polymers·Martina Rodriguez SalaFirouzeh Sabri
Aug 16, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Jella-Andrea AbrahamRudolf Merkel
Sep 27, 2018·ACS Applied Materials & Interfaces·Olivia V CangellarisMartha U Gillette
Nov 28, 2018·ACS Applied Materials & Interfaces·Geon KookHyunjoo J Lee
Jul 30, 2019·ACS Applied Materials & Interfaces·Gabriele TulliiMaria Rosa Antognazza

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