PMID: 7542782Jul 18, 1995Paper

Large secretory structures at the cell surface imaged with scanning force microscopy

Proceedings of the National Academy of Sciences of the United States of America
A Spudich, D Braunstein

Abstract

Scanning force microscopy was used to image rat basophilic leukemia (RBL-2H3) cell surfaces under different stimulation conditions that either permit or inhibit secretion. Cross-linking the surface IgE receptors with dinitrophenol-conjugated bovine serum albumin initiates secretion in RBL cells with concomitant spreading of the cell body. Structures at the cell surface approximately 1.5 microns in diameter relate to secretion both spatially and temporally. The position of these surface pits and their sizes suggest that they may be related to the dense-core granules positioned along the cytoskeletal filaments in detergent-extracted, unactivated RBL cell processes. Topographic scanning force microscopy images of RBL cell surfaces at 2, 5, and 35 min after activation show that these structures persist and change in cross-sectional profile with time after activation. These structures may be related to the membrane retrieval mechanism of cells after intense stimulation.

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Citations

Jun 20, 2008·The Journal of General Physiology·Tzu-Ming Wang, Donald W Hilgemann
Jan 16, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Adny H SilvaTânia B Creczynski-Pasa
Mar 11, 1996·FEBS Letters·D DunlapF Valtorta
Dec 20, 2002·Acta Physiologica Hungarica·S DamjanovichL Bene
Dec 17, 2002·Journal of Cell Science·Sophie Martin-VerdeauxUlrich Blank

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