Larger Than Life: Isolation and Genomic Characterization of a Jumbo Phage That Infects the Bacterial Plant Pathogen, Agrobacterium tumefaciens

Frontiers in Microbiology
Hedieh AttaiPamela J B Brown

Abstract

Agrobacterium tumefaciens is a plant pathogen that causes crown gall disease, leading to the damage of agriculturally-important crops. As part of an effort to discover new phages that can potentially be used as biocontrol agents to prevent crown gall disease, we isolated and characterized phage Atu_ph07 from Sawyer Creek in Springfield, MO, using the virulent Agrobacterium tumefaciens strain C58 as a host. After surveying its host range, we found that Atu_ph07 exclusively infects Agrobacterium tumefaciens. Time-lapse microscopy of A. tumefaciens cells subjected to infection at a multiplicity of infection (MOI) of 10 with Atu_ph07 reveals that lysis occurs within 3 h. Transmission electron microscopy (TEM) of virions shows that Atu_ph07 has a typical Myoviridae morphology with an icosahedral head, long tail, and tail fibers. The sequenced genome of Atu_ph07 is 490 kbp, defining it as a jumbo phage. The Atu_ph07 genome contains 714 open reading frames (ORFs), including 390 ORFs with no discernable homologs in other lineages (ORFans), 214 predicted conserved hypothetical proteins with no assigned function, and 110 predicted proteins with a functional annotation based on similarity to conserved proteins. The proteins with predicted...Continue Reading

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Citations

Dec 2, 2019·Archives of Virology·Yasuhiko KawatoToshihiro Nakai
Nov 27, 2018·Environmental Science and Pollution Research International·Hao WuYongsong Zhang
Dec 19, 2019·Frontiers in Microbiology·Mohammed ImamEdouard E Galyov

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Datasets Mentioned

BETA
MF403008

Methods Mentioned

BETA
PCR
transmission electron microscopy

Software Mentioned

SE
BLASTp
TMHMM
BLAST
ClustalW
PSI
SEQUEST
tRNAscan
Nikon Elements
ImageJ

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