Lariciresinol-4-O-β-D-glucopyranoside from the root of Isatis indigotica inhibits influenza A virus-induced pro-inflammatory response

Journal of Ethnopharmacology
Jing LiRongchang Chen

Abstract

Isatis indigotica is a traditional Chinese medicine. Its dried roots named "ban lan gen" in Chinese, are used for clinical treatment of virus infection, tumor, inflammation with a long history. However, its anti-influenza active ingredient and the underlying mechanism remain unclear. In this study, the anti-influenza and anti-inflammatory effects of a lignan glycoside: lariciresinol-4-O-β-D-glucopyranoside isolated from the root of I. indigotica on human alveolar epithelial cell line A549 infected with influenza A virus were investigated. Chemical and spectroscopic methods were employed to identify the structure of the lignan glycoside. Cytotoxicity of the lignan glycoside was analyzed using methylthiazolyltetrazolium (MTT) assay. The inhibitory activity against influenza virus of the lignan was determined by CPE inhibition assay. HEK-293 cells stably co-transfected with NF-κB responsive firefly luciferase and constitutively expressing GFP were employed for monitoring the effect of the lignan on NF-κB signal pathway activation. Nuclear export of viral ribonucleoprotein (RNP) complexes was monitored by indirect immunofluorescence. Quantitative real-time PCR was used to quantify the expression profiling of cytokines and chemokine...Continue Reading

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Citations

Oct 19, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shao-Ru ChenYing Wang
Jan 14, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhengtu LiZifeng Yang
Oct 17, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wenda GuanXiping Pan
Dec 5, 2018·Organic & Biomolecular Chemistry·Si-Fan LiuShao-Jiang Song
Aug 24, 2017·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Y ZhangY Yang
Apr 30, 2020·Journal of Biomolecular Structure & Dynamics·Loghman AlaeiHossein Derakhshankhah
Jul 18, 2020·Medicinal Research Reviews·Zhi-Jun ZhangRong-Tao Li
Sep 19, 2018·International Journal of Molecular Medicine·Wen YanXinhua Wang
Jan 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qinghua CuiLijun Rong
Feb 17, 2021·Phytotherapy Research : PTR·Sofi Imtiyaz AliShowkat Ul Nabi
Apr 20, 2021·Frontiers in Plant Science·Xiao ChenWansheng Chen
Jun 8, 2021·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Xin-Ya XuHong-Jie Zhang

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