PMID: 12770220May 29, 2003Paper

Larval and pupal induction and N-terminal amino acid sequence of lysozyme from Heliothis virescens

Journal of Insect Physiology
K T. Chung, D D Ourth

Abstract

Fifth instar larvae and prepupae of Heliothis virescens (tobacco budworm) were injected with live Enterobacter cloacae and bled at different times after vaccination. Immune pupal hemolymph showed a 54 times increase in lysozyme activity when compared with normal larval hemolymph, and an 11 times increase of lysozyme activity when compared with immune larval hemolymph. Lysozyme activity of the normal pupal hemolymph increased as greatly as did lysozyme activity of the immune larval hemolymph after metamorphosis. The pupal immune response with regard to lysozyme was much greater than the larval immune response in H. virescens. Lysozyme was purified by heat treatment at 100 degrees C and a chromatography series that included reverse-phase HPLC. The molecular mass of H. virescens lysozyme was approximately 16 kDa by SDS-PAGE which is greater than other insect lysozymes and chicken lysozyme. Amino acid sequence of the N-terminus showed that H. virescens lysozyme is 82% homologous with lysozyme of Manduca sexta and Galleria mellonella. CNBr cleavage of H. virescens lysozyme produced 11 and 6 kDa peptide fragments indicating that one methionine was present, which was also supported by amino acid analysis. However, methionine was locat...Continue Reading

References

Oct 1, 1992·Immunology Today·J A Hoffmann, C Hetru
Jan 1, 1991·Archives of Insect Biochemistry and Physiology·V W Russell, P E Dunn
Jan 29, 1988·Biochemical and Biophysical Research Communications·R NanbuS Natori
Apr 15, 1988·European Journal of Biochemistry·B GrindeP Jollès
Jul 15, 1973·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R F Powning, W J Davidson
Oct 14, 1968·Journal of Molecular Biology·M Inouye, A Tsugita
Mar 1, 1994·Insect Biochemistry and Molecular Biology·A B Mulnix, P E Dunn
Mar 27, 1996·Biochemical and Biophysical Research Communications·T D Lockey, D D Ourth
Sep 1, 1995·Developmental and Comparative Immunology·I MorishimaY Yamano
Feb 1, 1996·Insect Biochemistry and Molecular Biology·D KangH Steiner

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Citations

Jul 2, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Sangki Bae, Yonggyun Kim
Jun 19, 2010·Developmental and Comparative Immunology·Pawel MakMałgorzata Cytryńska
Aug 23, 2005·Biochemical and Biophysical Research Communications·Donald D OurthKyung T Chung
Dec 16, 2003·Biochemical and Biophysical Research Communications·Donald D Ourth, Kyung T Chung
Dec 30, 2006·Peptides·Małgorzata CytryńskaTeresa Jakubowicz
Jan 24, 2009·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Qian RenJinxing Wang
Apr 2, 2020·Archives of Insect Biochemistry and Physiology·Saraswathi SaraswathiManjulakumari Doddamane
Aug 1, 2006·Journal of Insect Physiology·Bin Li, Susan M Paskewitz

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