Larval fat body-specific gene expression in D. melanogaster

Developmental Genetics
J DeutschL Rat

Abstract

The Pl gene, together with the LSP-1 alpha, -1 beta, and -1 gamma, LSP-2, and P6 genes, is expressed exclusively in the larval fat body of D. melanogaster during the third instar. In vivo mapping of the cis-acting regulatory sequences of the P1 gene was carried out using hybrid constructs with three different reporter genes and a combination of transient and germline transformation assays. This revealed that regulatory elements involved in the setting up of the temporal and spatial specificities of transcription of the P1 gene are located in a short DNA region immediately upstream of the mRNA transcription start. This region includes an element that behaves as a fat-body transcriptional enhancer and element(s) required for ecdysone inducibility of transcription of the P1 gene.

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J A LepesantA Garen
Jan 1, 1978·Annual Review of Biochemistry·G R Wyatt, M L Pan
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C C Laurie, L F Stam
Jan 1, 1987·Annual Review of Biochemistry·M P Scott
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B D MarianiF C Kafatos
Oct 1, 1971·The Journal of Cell Biology·A Zweidler, L H Cohen
Mar 1, 1984·Developmental Biology·D PowellD B Roberts
Nov 1, 1983·Analytical Biochemistry·S Subramani, P J Southern
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·Y Nakanishi, A Garen
Mar 1, 1981·Molecular and Cellular Endocrinology·G Richards
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A GarenJ A Lepesant
Sep 1, 1970·Wilhelm Roux' Archiv Für Entwicklungsmechanik Der Organismen·Heinrich UrsprungNatalie Burroughs

❮ Previous
Next ❯

Citations

Jan 13, 2010·PloS One·Daniel J M Fernández-AyalaHoward T Jacobs
Nov 2, 2016·Autophagy·Anindita MukherjeeAndreas Jenny
Dec 13, 2005·Obesity Research·Zhihua JiangJohn P McNamara
Sep 6, 2017·Omics : a Journal of Integrative Biology·Manish KumarThottethodi Subrahmanya Keshava Prasad
Jun 22, 2019·Integrative and Comparative Biology·Allison M WelchAllison M Infante
Sep 3, 2016·Genetics·Nicole GreenErika R Geisbrecht
Jun 20, 2020·Autophagy·Ana MesquitaAndreas Jenny
Jul 20, 1990·Journal of Molecular Biology·F MaschatJ A Lepesant

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.